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A strain of Acremonium alternariae and its application

A technology of Acremonium acremonium and strains, applied in the field of Acremonium acremonium strains and their applications, and new microbial strains, which can solve the problems of increasing pesticide residues, polluting the environment, endangering human health and life, and achieving growth Quick, good insecticidal effect, and the effect of controlling the population of various pests

Active Publication Date: 2018-10-23
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical pesticides increases the amount of pesticide residues in agricultural products, seriously pollutes the environment, and endangers human health and life.

Method used

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  • A strain of Acremonium alternariae and its application
  • A strain of Acremonium alternariae and its application
  • A strain of Acremonium alternariae and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation of embodiment 1 bacterial strain

[0023] Disinfect the surface of the collected naturally infected Panonychus citrus mites with 70% alcohol, put them in a 25°C incubator for 3-5 days, and cultivate fresh spores on the surface of the insects; under sterile conditions, use Pick a small amount of fresh spores from the inoculation loop and streak on the PDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 25°C incubator for cultivation; pick out typical and spores after 3-4 days For colonies free of miscellaneous bacteria, use a transfer needle to pick up a small piece of medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and culture at a constant temperature of 25°C for 10 days.

Embodiment 2

[0024] The identification of embodiment 2 bacterial strains

[0025] (1) Morphological identification

[0026] The isolated (CQBBW8) strain was inoculated on a PDA plate and cultured at 25°C to observe the morphological characteristics of the colony, the sporulation structure, and the shape and size of the spores. see figure 1 , 2 3. The results show that the colony grows slowly. At 25°C, the diameter of PDA medium 10d is 40-45mm. The aerial hyphae are milky white and fluffy, and then slowly appear orange-red in the middle of the colony, moist and pungent. Sexual odor; the back of the colony is orange-yellow. Conidia are oblong, spindle-shaped, in chains or clusters, and are produced by chlamydospores. The conidiophores are scarce, and the conidia are spindle-shaped, smooth and transparent, with a size of 6.5-9.8×2.0-3.2μm. Most of them form sticky spore clusters, a few form dry short chains, and a large number of spores gather to produce a faint reddish brown.

[0027] ...

Embodiment 3

[0031] Example 3 CQBBW8 Determination of Indoor Insecticidal Activity to the 3rd Instar Larvae of Citrus Papilio Papilio

[0032] Scrape the CQBBW8 mycelium grown on the PDA plate for 7 days in sterile water with 0.02% Tween, shake and filter to collect the spore suspension, and configure it to a concentration of 2.47×10 6 10 mL of spore suspension per mL was evenly sprayed on the 3rd instar larvae of Citrus Papilio citrus swallowtail collected in the field, with 30 larvae for each treatment, and repeated 3 times. The samples were cultured with moisture at 25°C, and the control group was sprayed with 10 mL of sterile water containing 0.02% Tween, and the mortality rate was counted daily.

[0033] The experimental results showed that all the 3rd instar larvae of the citrus swallowtail died within 1 day of inoculation, and the mortality rate was 100%.

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Abstract

Provided is acremonium implicatum CQBBW8. The preservation number is CGMCC No.11440. The sequence homology between the rDNA-ITS sequence of the acremonium implicatum and the isolate LR2 of the acremonium implicatum reaches up to 98%. The acremonium implicatum CQBBW8 which is separated from citrus red mite bodies is good in production character and fast in growth, and the colony diameter can reach 5.083 cm after culture is performed for 15 days. Meanwhile, the killing activity on various pests is high, the pest killing effect is good, and the death rate of third instar larva of papilio xuthus linnaeus reaches up to 100%, the death rate of aphids reaches up to 100%, and the death rate of citrus red mites reaches up to 100% when the acremonium implicatum with 10<6> conidia / mL is used for killing the pests. An insecticidal fungus preparation produced through the acremonium implicatum effectively controls the colony quantity of various pests.

Description

technical field [0001] The invention relates to a new microbial strain, in particular to an acremonium acremonium strain and application thereof, belonging to the field of biological pesticides. technical background [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in agriculture as an increase in production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (i.e. resistance to pesticides, residence of pesticide residues, No matter how rampant Resurgence is, it has also brought a series of ecological and environmental problems. Long-term extensive use of chemical pesticides has led to an increase in drug-resistant pests. Especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, and Spodoptera litura to pyrethroids and organophosphorus ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/02A01P7/04A01P3/00A01P1/00A01N59/20A01N47/14A01N43/54A01N43/713A01N43/12
Inventor 胡军华王娟姚廷山刘浩强李鸿筠冉春
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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