A microsatellite multiplex pcr method for paternity identification of oyster oyster

A technology for paternity testing and oyster growth, which is applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of easy loss and change of physical markers, low reliability, etc., and achieve the effect of improving analysis accuracy and experimental efficiency
CN105441576BActive Publication Date: 2019-08-16OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2019-08-16

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Abstract

The invention discloses a microsatellite multiplex-PCR (Polymerase Chain Reaction) method for carrying out a paternity testing on crassostrea gigas. The microsatellite multiplex-PCR method comprises the following steps of extracting parent and offspring DNA (Deoxyribose Nucleic Acid); carrying out multiplex-PCR amplification on extracted DNA samples by using 18 microsatellite sites and M13 (-21) universal primers with fluorescent marks; detecting a fluorescent signal of an amplified product, and obtaining parent and offspring gene type data; carrying out genetic relationship identification on the parent and the offspring. The microsatellite multiplex-PCR method disclosed by the invention verifies that two groups of multiplex-PCR combinations are used for carrying out the paternity testing on 12 crassostrea gigas full-sib families, so that the identification success rate is up to 100 percent; a set of DNA mark-based paternity testing technology which is economic and effective and is high in accuracy is provided for the crassostrea gigas; by a genealogical relationship record obtained through microsatellite mark identification, producing places of individual crassostrea gigas can be traced back, so that a tracking and source tracing technology of crassostrea gigas products can be established, and powerful technical support can be provided for solving food safety problems which are increasingly concerned by people in nowadays society.
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Description

technical field

[0001] The invention relates to a method for paternity identification of aquatic animals, in particular to a method for paternity identification of long oyster by using a microsatellite multiplex PCR method. Background technique

[0002] The microsatellite marker method has the characteristics of rich polymorphism, high heterozygosity, good stability, following Mendelian segregation law, co-dominant inheritance, and easy PCR amplification. It is one of the most popular molecular markers today. In paternity testing, compared with SNP markers, using microsatellite markers requires fewer loci, but the relative identification power can reach more than 5 times; microsatellite marker method does not require special equipment, and it is easy to operate, easy to detect, time-saving and efficient and high accuracy features.

[0003] Using microsatellite markers for paternity identification, the traditional analysis method is to type the PCR amplification products on ...

Claims

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