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Microorganism culturing medium and application thereof

A technology of microbial culture medium and basal medium, applied in the field of culture medium, can solve the problems of killing microorganisms, slow detection results, false negatives, etc., to reduce growth inhibition, reduce inhibition, improve detection speed and accuracy degree of effect

Inactive Publication Date: 2016-04-13
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibiotics, such as penicillin and streptomycin, are added to most cell samples in the early stage of culture, and the presence of antibiotics will inhibit the growth of microorganisms in the cell samples to a certain extent, but will not really kill the microorganisms, resulting in detection Slow and even false negative results

Method used

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  • Microorganism culturing medium and application thereof
  • Microorganism culturing medium and application thereof
  • Microorganism culturing medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of microbial culture medium

[0034] a) First accurately weigh 35g dry agar powder (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 1g sodium chloride, 3g yeast extract (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), dissolve them in 1000ml of distilled water, and stir well , Seal and autoclave at 121℃ for 30min;

[0035] b) After the sterilization, when the temperature of the medium drops to 50℃, add 5g glucose, 3ml β-lactam antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 3ml after sieving and sterilizing in the ultra-clean workbench Aminoglycoside antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.) and 300μL phenol red, stir well;

[0036] c) After the temperature of the culture medium drops to 40°C, add 5ml calf serum, 1.5g B vitamins, 1.5g vitamin D, 1ml non-essential amino acids (purchased from Yingwei Jieji Trading Co., Ltd.), and stir well to obtain microorganisms C...

Embodiment 2

[0039] Preparation of microbial culture medium

[0040] a) First accurately weigh 25g dry agar powder (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 3g sodium chloride, 1g yeast extract (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), dissolve them in 1000ml of distilled water, and stir well , Seal and autoclave at 121℃ for 30min;

[0041] b) After the sterilization is completed, wait for the medium temperature to drop to 50°C, and add 10g glucose, 1ml β-lactam antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 5ml after sieving and sterilizing into the ultra-clean workbench Aminoglycoside antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.) and 500μL phenol red, stir well;

[0042] c) After the temperature of the culture medium drops to 40℃, add 1ml calf serum, 3g B vitamins, 1g vitamin D, and 5mL non-essential amino acids (purchased from Yingwei Jieji Trading Co., Ltd.), stir well to obtain mi...

Embodiment 3

[0045] Preparation of microbial culture medium

[0046] a) First accurately weigh 40g dry agar powder (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 0.5g sodium chloride, 5g yeast extract (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), dissolve in 1000ml of distilled water, and stir. Evenly, seal and autoclave at 121℃ for 30min;

[0047] b) After the sterilization, when the temperature of the medium drops to 50℃, add 1g glucose, 5ml β-lactam antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.), 1ml after sieving and sterilizing in the ultra-clean workbench Aminoglycoside antagonist (purchased from Guangdong Huankai Microbial Technology Co., Ltd.) and 200μL phenol red, stir well;

[0048] c) After the temperature of the culture medium drops to 40℃, add 7ml calf serum, 1g B vitamins, 3g vitamin D, 0.5mL non-essential amino acids (purchased from Yingwei Jieji Trading Co., Ltd.), stir well to obtain microbial culture base; ...

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Abstract

The invention belongs to the field of culturing media, and particularly relates to a microorganism culturing medium and an application thereof. The culturing medium comprises 800-1,300 g of a basic culturing medium, 0.5-7 mL of serum, 1-5 mL of beta-lactam antagonist and 1-5 mL of aminoglycoside antagonist. The culturing medium comprises the basic culturing medium, the serum, the beta-lactam antagonist and the aminoglycoside antagonist; due to the synergistic effect of the components, it is guaranteed that microorganisms can well grow in the culturing medium, the inhibition effect of antibiotics on the growth of the microorganisms can be reduced, the microorganism detection speed in a cell sample is increased accordingly, and the microorganism detection accuracy in the cell sample is improved. The test result indicates that the microorganisms can keep the good growing situation in the culturing medium, and the culturing medium can effectively reduce the inhibition effect of antibiotics on the growth of the microorganisms.

Description

Technical field [0001] The invention belongs to the field of culture media, and particularly relates to a microorganism culture medium and its application. Background technique [0002] With the advancement of science and technology, people's research on cell and molecular medicine has been intensified. Currently, cell therapy has been successfully applied clinically, including autoimmune cell therapy and autologous stem cell therapy. For cancer patients, autoimmune cell therapy has brought them a new health gospel; for some patients with intractable diseases, autologous stem cell therapy has brought new impetus to their lives. Therefore, cell therapy has very important significance in future life science technology research. [0003] The so-called cell therapy refers to the use of certain cells with specific functions, which are obtained by bioengineering methods and then proliferated and cultured in vitro, so that these cells have therapeutic effects such as enhancing immunity,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12Q1/04
CPCC12N1/20C12N1/14C12Q1/04
Inventor 陈海佳王一飞葛啸虎黎娟妹
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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