Blood screening kit, blood screening method and blood screening device

A blood screening and kit technology, which can be used in microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc. Shorten the detection window period and improve the effect of the detection cycle

Inactive Publication Date: 2016-04-27
天津华大基因科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Although these technologies have greatly shortened the window period and reduced the probability of blood-borne viral diseases, due to the shortcomings of the PCR method alone, for example, it may not be able to detect trace pathogens well, and the detection cycle is relatively long
Moreover, at present domestic blood source screening is difficult to comprehensively screen all kinds of viruses at once.

Method used

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  • Blood screening kit, blood screening method and blood screening device
  • Blood screening kit, blood screening method and blood screening device
  • Blood screening kit, blood screening method and blood screening device

Examples

Experimental program
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Embodiment 1

[0035] The following is based on the blood sample obtained from Inner Mongolia Maternal and Child Health Hospital, illustrating the blood screening method of the present invention, including the following steps: designing corresponding primers for the conserved regions of the nucleotide sequences of various pathogens to be tested, each pair; plasma / serum Nucleic acid extraction; PCR amplification to obtain amplified products containing specific gene sequences; amplified product equal pooling; DNA library construction; high-throughput sequencing; data analysis to determine the type of infectious pathogen. In this example, BGISEQ100 high-throughput sequencing technology is used to detect HBV, HCV, HIV, TP, CMV and HTLV samples in patient plasma, and the specific operation steps are as follows:

[0036] 1. First, design the respective primers according to the genome sequences of HBV, HCV, HIV, TP, CMV and HTLV pathogens, and consider and test so that part or all of the primer pair...

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Abstract

The invention provides a virus detecting kit, a blood screening method and a blood screening device. The virus detecting kit comprises at least two of six pairs of primers: SEQ ID NO:1 and SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10 as well as SEQ ID NO:11 and SEQ ID NO:12. By the virus detecting kit, the blood screening method or the blood screening device, samples low in viral load can be detected, and accurate detecting results, low detection cost and short consumed time are achieved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a kit for screening viruses in blood, a blood screening method and a device thereof. Background technique [0002] Various serious infectious diseases such as hepatitis B, hepatitis C, AIDS and syphilis can be transmitted through blood. To ensure the health of workers, strict screening of pathogens must be carried out on blood sources. In recent years, with the development of biomedicine and testing technology, the safety of blood transfusion and blood products has been greatly improved, but the residual risk of viral diseases transmitted by blood is still high. [0003] Currently, Enzyme-Linked Immunosorbnent Assay (ELISA, Enzyme-Linked Immunosorbnent Assay) is widely used in clinical blood screening. With the application of monoclonal antibodies and the establishment of a more sensitive luminescence platform, its sensitivity and specificity have been continuously improved ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12M1/34C12R1/93
Inventor 姚雪周加利栗东方吴红龙
Owner 天津华大基因科技有限公司
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