Saffron crocus culture medium for increasing content of crocin

A technology of crocin and culture medium, which is applied in the field of plant tissue culture, can solve the problems of low crocin content, achieve the effects of improving enzyme activity, increasing content, and promoting synthesis

Inactive Publication Date: 2016-05-04
FOSHAN JINLANLING EDUCATION TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main active ingredient of saffron is crocin, but the live saffron plant can only extract a small amount of crocin in the stigma, which is difficult to meet the demand of the market. For this reason, it is necessary to provide a technology that can extract crocin from the saffron callus, however The content of crocin in the callus in the current tissue culture technology is extremely low

Method used

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  • Saffron crocus culture medium for increasing content of crocin
  • Saffron crocus culture medium for increasing content of crocin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of a saffron medium for increasing the content of saffron.

[0014] According to the composition and dosage of MS medium shown in Table 1 and the composition and dosage of saffron medium for increasing crocin content shown in Table 2, the saffron medium for increasing crocin content according to the present invention is prepared.

[0015] Weigh the components of MS medium, 6-BA (6-benzyl adenine), 2,4-D (2,4-dichlorophenoxyacetic acid), lanthanum nitrate and sucrose with a balance, and place them in a conical flask Add an appropriate amount of distilled water, stir to dissolve, adjust the pH to 6.0 with HCL and KOH, and make up to 1000 mL. Seal the triangular flask, place it in an autoclave, sterilize it at 121°C for 20 minutes, and then place it in an ultra-clean workbench for natural cooling and set aside.

[0016] When it is cooled to 50-60°C, the culture medium is divided into tissue culture flasks, allowed to cool, and the tissue culture fla...

Embodiment 2

[0021] Example 2: The test of the saffron culture medium for increasing the saffron content.

[0022] According to the method described in Example 1, the saffron medium for improving the content of saffron was prepared and used for later use.

[0023] In an ultra-clean bench, remove the normally healthy, non-browning, non-vitrified saffron callus from the value-added medium and inoculate the saffron callus with a sterile scalpel in 0.5-1 cm pieces and inoculate In the prepared saffron medium for improving the content of crocin, the inoculated biomass was 6g / L, and it was placed in an artificial climate box for cultivation. The cultivation environment conditions were, light / dark time: 14 / 10 hours, and the cultivation temperature was 23°C.

[0024] After cultivating for 30 days, the callus was taken out, and the content of crocin in the callus was extracted, reaching 1.02g\L, which was 0.3g\L higher than the callus content of the control group, which was 0.72g\L. More than 30%...

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Abstract

The invention provides a saffron crocus culture medium for increasing the content of crocin, and belongs to the technical field of plant tissue culture. An MS culture medium is used as a basic culture medium, and 6-BA(6-benzyladenine), 2,4-D(2,4-dichlorphenoxyacetic acid), lanthanum nitrate and saccharose are added. The culture medium is a liquid culture medium, rare earth elements are added, and the content of the crocin in saffron crocus callus tissue is increased by utilizing regulating characteristics of the rare earth elements for plants.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a saffron culture medium for improving the content of saffron. Background technique [0002] Saffron is a kind of precious Chinese herbal medicine. Because it is only used as a stigma, the yield is extremely low. At the same time, because its resources are extremely limited, but it is widely used, making it expensive. It has always been known as "plant gold". Research is extremely important. [0003] The main active ingredient of saffron is saffron, but living saffron plants can only extract a small amount of saffron in the stigma, which is difficult to meet the needs of the market. For this reason, it is necessary to provide a technology that can extract saffron from saffron callus. However, The content of crocin in callus is extremely low in current tissue culture techniques. SUMMARY OF THE INVENTION [0004] Based on the above problems in the prior art, the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 何志铿
Owner FOSHAN JINLANLING EDUCATION TECH
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