Method for making permanent glass slide specimen of bryophyte

Abstract

The invention relates to a method for making a permanent glass slide specimen of bryophyte. Gum Arabic, double distilled water, glycerin and chloral hydrate are mixed with stirring at normal temperature, and a liquid mounting medium is prepared; moss leaves or plants are placed in the double distilled water, after infiltration, the moss leaves or plants are placed in the center of a slide glass with a droplet of double distilled water, and the moss leaves or plants are observed under a 10x microscope for adjusting shapes; a piece of filter paper is used for absorbing double distilled water which is 1 millimeter or more far away from the specimen, till the circumference of the moss specimen is moistened without excessive double distilled water; a glass rod is used for dipping 1-2 droplets of the mounting medium, and the droplets are dropped on the moss specimen, a piece of cover glass is covered without bubbles contained, the specimen is placed at normal temperature till the mounting medium is dried and can fix the specimen without movement, a label is plastered on the slide glass, and the permanent glass slide specimen of bryophyte is prepared. Compared with prior art, the produced specimen has the advantages of good transparency, simple method, long preservation time limit, and good preservation effect of original state of the specimen.

Description

technical field [0001] The invention relates to a method for making a biological permanent glass slide, in particular to a method for making a bryophyte permanent glass slide specimen. Background technique [0002] In natural science research, bryophyte species identification, biological experiment teaching and extracurricular scientific and technological activities, especially in the bryophyte species identification and morphological research, it is necessary to make a large number of moss microscopic slide specimens; moss slides can be divided into temporary glass slides. At present, the production of temporary slides generally uses water directly as a floating agent, and then directly observes after covering with a cover glass. However, the temporary slides made in this way can only be stored for a short period of time, and the water will volatilize after a long time , affect further observation, and cannot be further sealed for storage; permanent glass slides require rep...

AI Technical Summary

Problems solved by technology

[0002] In natural science research, bryophyte species identification, biological experiment teaching and extracurricular scientific and technological activities, especially in the bryophyte species identification and morphological research, it is necessary to make a large number of moss microscopic slide specimens; moss slides can be divided into temporary glass slides. At present, the production of temporary slides generally uses water directly as a floating agent, and then directly observes after covering with a cover glass. However, the temporary slides made in this way can only be stored for a short period of time, and the water will volatilize after a long time , affect further observation, and cannot be further sealed for storage; permanent glass slides require repeated observation and long-term storage, so the mounting medium used is required to be stored for a long time, not deteriorated, has strong adhesion, and the refractive index is the same as that used for slides, The refractive index of the cover glass etc. is similar

At present, paraffin-wax technology is usually used for sealing, but the process of paraffin-wax technology is cumbersome, the production time is long, and it is easy to be restricted by conditions. Many chemical reagents are used in the production process, and some reagents are poisonous to humans. It becomes brittle, hard or deformed, which affects the accuracy of observation, and because the water content in the cells of bryophytes is high, the tissue is easy to shrink and deform during the dehydration process, so it is not suitable for bryophytes

[0003] Chinese patent CN101571626A discloses a method for transparent sealing of biological microscopic slide specimens, using novolac as a mounting agent to seal the specimens, and this method uses varnish for sealing with high light transmittance, high quality and low price, and easy to obtain materials , but the phenolic novolak is brittle after drying, and it is easy to turn yellow under the action of ultraviolet light, so it will affect the follow-up observation and specimen protection