The invention provides a sea cucumber and oyster peptide chewable tablet for nourishing liver, protecting kidney and resisting fatigue and a preparation method thereof. The chewable tablet is preparedfrom the following components: sea cucumber peptide, oyster peptide, casein phosphopeptide, whey protein concentrate, fruit and vegetable powder, lactose, xylooligosaccharide, resistant dextrin, fructo-oligosaccharide, chitosan oligosaccharide, vitamin C, erythritol, sorbitol, citric acid, DL-malic acid, magnesium stearate and targeted slow-release biocompatible microcapsules. The raw materials are natural, various in components, light in concentration and convenient to take; the chewable tablet has the advantages that the requirements of macroelements, macroelements and microelements are met; health is taken as a central idea, various nutrient requirements of teenagers are met, regulation substances with physiological functions and the regulation effect of'peptides' on cells are used asauxiliary materials, the disadvantages of the existing products are broken through, and a physiological function which is convenient to use, safe, reliable, capable of rapidly and timely conveying nutrients to each cell of each tissue of the whole body and capable of regulating and controlling cell growth, replication, reproduction and metabolism is provided.