Drug-carrying TAT-CS modified carbon nanotube and preparation method and application thereof

A technology of TAT-CS and carbon nanotubes, which is applied in the field of tumor treatment and biomaterials, can solve the problems of large usage and unsatisfactory effects, and achieve the effect of preventing the destruction of the surface structure, avoiding the impact, and improving the dispersion

Inactive Publication Date: 2016-05-04
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a drug-loaded TAT-CS modified carbon nanotube and its preparation method

Method used

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  • Drug-carrying TAT-CS modified carbon nanotube and preparation method and application thereof
  • Drug-carrying TAT-CS modified carbon nanotube and preparation method and application thereof
  • Drug-carrying TAT-CS modified carbon nanotube and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] A drug-loaded TAT-CS modified carbon nanotube, the amino acid sequence of the TAT polypeptide is "N"-CYGRKKRRQRRR-"C", the CS is low molecular weight chitosan (5000-8000), and the bifunctional coupling agent Under the action of 3-(2-pyridyldimercapto)propionic acid N-hydroxysuccinimide ester (SPDP), the covalent cross-linking is TAT-CS, and the carbon nanotubes are original multi-walled carbon nanotubes with a purity> 98%, tube diameter < 8nm, tube length 0.5-2 μm, add the carbon nanotubes into ultrapure water for 30 minutes of ultrasonication, the concentration is 1 mg / ml. Prepare doxorubicin (DOX) solution with a concentration of 6 mg / ml, mix the carbon nanotube solution with the DOX solution at a mass ratio of DOX:carbon nanotubes=3:1, and shake overnight at room temperature. Add TAT-CS solution, shake at room temperature for 16 hours, dialyze the dialysis bag overnight, and dialyze twice continuously, the molecular weight cut-off of the dialysis bag is 3500. Calcul...

Embodiment 2

[0027] A drug-loaded TAT-CS modified carbon nanotube, the carbon nanotube is a carboxylated multi-walled carbon nanotube with a purity > 98%, a -COOH content of 3.86%, a tube diameter < 8nm, and a tube length of 0.5-2 μm. The carbon nanotubes were added into ultrapure water for 30 minutes of ultrasonication, with a concentration of 1 mg / ml. Prepare a DOX solution with a concentration of 6 mg / ml, mix the carbon nanotube solution and adriamycin solution at a mass ratio of DOX:carbon nanotube=3:1, and stir overnight at room temperature. Add TAT-CS solution, shake at room temperature for 16 hours, dialyze the dialysis bag overnight, and dialyze twice continuously, the molecular weight cut-off of the dialysis bag is 3500. Calculate encapsulation efficiency and drug loading.

Embodiment 3

[0029] A drug-loaded TAT-CS modified carbon nanotube, the carbon nanotube is an original multi-walled carbon nanotube with a purity > 98%, a tube diameter < 8nm, and a tube length of 0.5-2 μm. Add the carbon nanotube to ultrapure water Ultrasound was carried out for 30min in the middle, the concentration was 1mg / ml. Paclitaxel (Paclitaxel) solution was prepared at a concentration of 6 mg / ml, and the carbon nanotube solution was mixed with the Paclitaxel solution at a mass ratio of Paclitaxel:carbon nanotubes=4:1, and stirred overnight at room temperature. Add TAT-CS solution, shake at room temperature for 16 hours, dialyze the dialysis bag overnight, and dialyze twice continuously, the molecular weight cut-off of the dialysis bag is 3500. Calculate encapsulation efficiency and drug loading.

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Abstract

The invention discloses a drug-carrying TAT-CS modified carbon nanotube and a preparation method and application thereof. The drug-carrying TAT-CS modified carbon nanotube is prepared from an antitumor drug and a TAT-CS modified multiwalled carbon nanotube. The multiwalled carbon nanotube is dissolved in ultrapure water and dispersed evenly in an ultrasonic dispersion mode, then the antitumor drug is added, the mixture is shaken at the room temperature and placed overnight, a TAT-CS solution is added, the mixture is shaken at the room temperature and dialyzed, and the drug-carrying TAT-CS modified carbon nanotube is obtained. By modifying the carbon nanotube with TAT-CS, the dispersity and biosecurity of the carbon nanotube are further improved, the cell intake rate of the carbon nanotube is further increased, the bioavailability of the drug can be increased, the dosage of the drug can be reduced, and thus the problems that the antitumor drug is strong in toxic and side effect and short in half-life period are solved; meanwhile, combined treatment is performed by utilizing the photothermal effect of the carbon nanotube. According to the combined treatment method, the same nanomaterial serves as a carrier and an optothermal agent, and therefore under the same treatment effect, the toxic and side effect of the drug on normal tissue of the body can be greatly reduced by reducing the dosage of the chemotherapeutic drug, and the treatment effect can be improved through combined thermal treatment.

Description

technical field [0001] The invention belongs to the fields of biological materials and tumor treatment, and in particular relates to a tumor killing method and application of TAT-CS modified carbon nanotube-based chemotherapy combined with hyperthermia. Background technique [0002] In the past ten years, although the three main treatment methods for tumors: surgery, radiotherapy and chemotherapy have been continuously improved, the cure rate of cancer patients is still low, and there are many problems in these traditional treatment methods, especially surgery, radiotherapy and chemotherapy. The curative effect of radiotherapy and chemotherapy on advanced malignant tumors is still not optimistic. It cannot avoid the metastasis and recurrence of cancer cells, and usually seriously reduces the immunity of patients, forming a vicious circle. Therefore, while improving traditional treatment methods, researchers are also constantly seeking new and effective treatment methods or b...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/42A61K47/02A61K45/06A61K31/704A61K31/337A61K41/00A61P35/00
CPCA61K47/36A61K9/0092A61K31/337A61K31/704A61K41/0052A61K45/06A61K47/02A61K47/42A61K2300/00
Inventor 冷希岗董霞刘兰霞朱敦皖张海玲
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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