Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method

A solid medium, Cordyceps sinensis technology, applied in the directions of microorganism-based methods, fungi, biochemical equipment and methods, etc., can solve the problems of long solid culture time, affecting product palatability, chemical residues, etc. It takes time, good loose air permeability, good absorption effect

Active Publication Date: 2016-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these auxiliary materials are not normal ingredients. The silkworm chrysalis has a special smell that will affect the palatability of the product, and some will cause chemical residues
In addition, because Cordyceps sinensis is an aerobic bacterium, its

Method used

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  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method
  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method
  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0064] The preparation method of Cordyceps sinensis secondary seed liquid (secondary bacterial species) is as follows:

[0065] Under aseptic operation conditions, pick the slant moss of Cordyceps sinensis ---- Cordycepssinensis slant strains and inoculate them on a PDA plate, culture at 15~25°C for 5 days to obtain the activated moss of Cordyceps sinensis; pick Cordyceps sinensis The activated bacterial lawn was inoculated into PDA liquid medium and cultured at 18-22°C with shaking (100-200r / min) for 5 days to obtain the first-level seed solution of Cordyceps sinensis (first-level seed bacteria solution); press 10% (v / v) ) Inoculum amount: Inoculate the first-level seed bacterial solution in the PDA liquid culture solution, and incubate at 18-22°C with shaking (100-200r / min) for 5 days to obtain the second-level bacterial species (secondary seed solution of Cordyceps sinensis), and set aside ( Put it at 4°C for use).

[0066] In this secondary strain, the content of Cordycepssine...

Example Embodiment

[0067] Example 1,

[0068] 1. Cordyceps mycelium solid culture medium and preparation method, the formula of the culture medium is composed of the following ingredients by weight percentage:

[0069]

[0070] The preparation method of the culture medium is implemented as follows:

[0071] ① Rinse non-glutinous yellow millet with tap water as the main ingredient of the culture medium;

[0072] ②. The washed peeled potatoes and rabbit meat are crushed and homogenized to obtain slurry;

[0073] ③. Mix the slurry prepared from the main ingredients and auxiliary ingredients after washing with sucrose and water, and cook the mature rice in an automatic rice cooker;

[0074] ④. Dispense the cooked rice into 15 cm petri dishes, sterilize at 121°C for 20 minutes, and cool down to obtain a solid medium for use.

[0075] 2. Cordyceps sinensis mycelium solid culture method, followed by the following steps:

[0076] ① Spray 75% (vol%) alcohol on the inner wall of the incubator first, and then start the...

Example Embodiment

[0083] Example 2,

[0084] The formula of Cordyceps mycelium solid culture medium consists of the following ingredients by weight:

[0085]

[0086] The rest of the content is the same as in Example 1 (that is, the preparation method of solid medium and the solid culture method of cordyceps mycelium are equivalent to Example 1.)

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Abstract

The invention discloses a cordyceps militaris mycelium solid culture medium. The solid culture medium comprises the following components in percentage by weight: 30%-50% of main materials with the particle sizes less than or equal to 2mm, 0.1%-15% of livestock, 0.1%-15% of vegetables, 0.1%-15% of saccharides and the balance of water, wherein the main materials are non-glutinous cereals or beans, the livestock and the vegetables are auxiliary materials, and the saccharides are monosaccharides or disaccharides. The invention further simultaneously discloses a preparation method of the cordyceps militaris mycelium solid culture medium and a food material-based cordyceps militaris mycelium solid culture method utilizing the solid culture medium. By taking the food materials as the main materials and the auxiliary materials, the solid culture medium has relatively large specific surface area and good loosening breathability, and good nutritional conditions and physical space can be provided for the growth of cordyceps militaris mycelia.

Description

technical field [0001] The invention belongs to the field of biological material cultivation methods, and in particular relates to a solid culture medium and a cultivation method for Cordyceps sinensis mycelia. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is a complex of insects and fungi formed by the larvae of the Lepidoptera bat moth family Batmoth genus parasitized by the Chinese spore (Hirsutellasinensis). Dating back thousands of years. According to previous editions of the "Pharmacopoeia of the People's Republic of China", its main effects are to nourish the kidney and lung, stop bleeding and resolve phlegm; it is used to treat kidney deficiency and essence deficiency, impotence and nocturnal emission, soreness of the waist and knees, chronic cough and dyspnea due to deficiency, and hemoptysis due to fatigue. Modern medical and pharmacological studies have proved that Cordyceps sinensis has a variety of therapeutic and nourishing health ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 方萍谢媛
Owner ZHEJIANG UNIV
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