Tobacco retrotransposon gene Ntrt1 and use thereof

A technology of retrotransposon and tobacco, applied in the direction of genetic engineering, plant gene improvement, application, etc.

Active Publication Date: 2016-05-04
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although bioinformatics methods can be used to predict functional genes, the final function of genes still depends on biological identification.

Method used

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  • Tobacco retrotransposon gene Ntrt1 and use thereof
  • Tobacco retrotransposon gene Ntrt1 and use thereof
  • Tobacco retrotransposon gene Ntrt1 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Materials and methods

[0018] 1.1 Experimental materials and instruments

[0019] 1.1.1 Plant material: Tobacco cultivar "Guiyan No. 1".

[0020] 1.1.2 Main instrument: BDSMART TM RACE cDNA Amplification Kit, dNTP, AMV(ReverseTranscriptaseXL), oligod(T) 18 , Ribonuclease Inhibitor, DNAMarkerDL2000, pMD18-T vector, etc. were purchased from Takara Company; TransTaq DNA polymerase HiFi, Escherichia coli (Escherichiacoli) DH5α were purchased from Beijing Quanshijin Biotechnology Co., Ltd.; the Trizol kit used for extracting total RNA was purchased from Invitrogen Company; ampicillin Penicillin, DEPC, CaCl 2 Purchased from Shanghai Sangon Bioengineering Co., Ltd.; DNAGelExtraction kit was purchased from Omega Company, and PCR primers were synthesized by Shanghai Yingjun Company.

[0021] 1.2 Test method

[0022] 1.2.1 Extraction of total RNA from tobacco leaves

[0023] Take about 0.1 g of fresh tobacco leaves and place them in an RNase-free mortar, add liquid nitr...

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Abstract

The invention discloses a tobacco retrotransposon gene Ntrt1. The tobacco retrotransposon gene Ntrt1 has a nucleotide sequence represented by SEQ ID NO:1. A protein encoded by the gene is characterized in that the protein has amino acid sequences represented by SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4; and the protein is used for adjusting the height of tobacco plants.

Description

technical field [0001] The invention relates to a tobacco retrotransposon gene Ntrt1 and its application, belonging to the field of plant genetic engineering. Background technique [0002] Retrotransposon (retrotransposon) is a kind of mobile genetic element in eukaryotic genome, and its transposition depends on the reverse transcription reaction of reverse transcriptase, and proceeds in the form of DNA-RNA-DNA. Retrotransposons were first discovered in animals and yeast, and they also widely exist in plant genomes, and their proportions in the genome are also different. For example, the proportion of retrotransposons in rice genes is about 18%, while it reaches 50%-80% in maize, and as high as 90% in wheat. According to their structure, retrotransposons can be divided into two categories: long terminal repeat retrotransposons (longterminalrepeats, LTRs) and non-longterminal repeat retrotransposons (non-longterminalrepeats, non-LTRs). LTR has a pair of highly similar seque...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 郭玉双任学良余婧
Owner GUIZHOU TOBACCO SCI RES INST
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