Method for rapidly detecting content of nicotine and cotinine in meconium of newborn
A newborn, nicotine technology, applied in the field of mass spectrometry detection, to avoid human error, high detection accuracy, improve the effect of determination accuracy and accuracy
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[0010] A method for rapidly detecting the content of nicotine and cotinine in neonatal meconium, the steps of which are: (1) take a sample of neonatal meconium with a medical cotton swab; The internal standard methanol solution containing nicotine and cotinine isotopes is used as an extraction ionization reagent to ionize the sample and generate a spray so that the center line of the mass spectrum is parallel to the toothpick with a distance of 0.5-5cm; (3) Turn on the mass spectrometry system and set it to positive ion mode, the ionization voltage is 3.5kV, the capillary temperature is 100 degrees, multiple reaction monitoring scan mode detection, nicotine: 163.2-130.2; cotinine: 177.1-80.2, other parameters are automatically optimized by the mass spectrometer system.
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