Method for rapidly detecting content of nicotine and cotinine in meconium of newborn

Abstract

The invention discloses a method for rapidly detecting the content of nicotine and cotinine in meconium of a newborn. The method comprises the steps: extracting a meconium sample of the newborn by using a medical cotton swab; dropwise adding an isotopic internal standard methanol solution containing the nicotine and cotinine to a part, away from the sample by 0.5mm to 1.5mm, on the medical cotton swab as an extracting ionization reagent, so as to ionize the sample and generate atomization, and enabling a mass-spectrum centerline to be parallel to a toothpick at the distance of 0.5cm to 5cm; starting up a mass spectroscopy system, and setting as a cation mode. The method disclosed by the invention has the advantages that a pretreatment process for the sample is simplified, sample pretreatment such as extraction and chromatographic separation is not carried out, and the newborn is subjected to direct ionization mass spectroscopy through swabbing an electrospray mass spectrum by adopting the medical cotton swab, so that the whole analysis process is greatly simplified; the medical cotton swab is clean and hygienic and is particularly applicable to clinical large-batch analysis. Sample pretreatment steps are simplified, and direct ionization is carried out, so that the correctness and accuracy of assaying are greatly improved.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry detection, in particular to a method for rapidly detecting the contents of nicotine and cotinine in meconium of newborns by direct mass spectrometry based on medical cotton swabs. Background technique [0002] Smoking will bring a series of health problems, and smoking during pregnancy or passive smoking is more harmful. Domestic and foreign studies have shown that smoking during pregnancy or passive smoking is harmful to pregnant women and fetuses. Exposure to various cigarette smoke is harmful to pregnant women and fetuses. The risk factors that affect are constantly being discovered. Laurberg of Ringkoebing Hospital in Denmark and his colleagues observed pregnant women and their babies during the hospitalization period. Smoking increases the risk of brain damage in children due to iodine deficiency; Saarland University in Germany reported that smoking in pregnant women can easily cause...

AI Technical Summary

Problems solved by technology

At present, there are few reports on the detection methods of nicotine and cotinine levels in meconium, so there is

Images