Manufacturing method for artificial cymbidium seeds

A technology of artificial seeds and production methods, applied in horticultural methods, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of slow reproduction speed, low degree of mechanization, cumbersome operation steps, etc., to reduce production costs, improve The effect of high production efficiency and culture germination rate

Inactive Publication Date: 2016-05-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Cymbidium is mostly a hybrid variety, the natural seed setting rate of the seeds is quite low, and the seeds are not fully developed and have no endosperm. Under normal circumstances, it is extremely difficult to germinate, and sterile sowing techniques are required to germinate. In addition, the branching ability of Cymbidium is weak, so it is difficult to reproduce, and the propagation speed is slow, which is far from meeting the requirements of commercial production, and tissue culture technology has become an important means of large-scale reproduction and production of Cymbidium (Chen Zhang, 2004)
Although there are many reports on the rapid propagation system of Cymbidium in China, most of them focus on the proliferation of sterile seedlings, etc., but there is no report on the proliferation and artificial seed embedding of Cymbidium by using protocorm-like liquid shock culture.
[0003] The conventional tissue culture seedlings of Cymbidium are produced in factories, usually adopting the technology of adventitious bud proliferation and rooting culture. The operation steps are cumbersome, the growth cycle is long, the degree of mechanization is low, labor and time-consuming, high energy consumption, and high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Carry out the following operations with the Cymbidium variety '4073' as material.

[0025] (1) Test material: protocorm-like induced stem tip of Cymbidium grandiflora, inoculated to 1 / 2MS+NAA0.5mg·L - 1 + coconut milk 100ml·L -1 + sucrose 30g·L -1 +Agar 6.0g·L -1 +Activated carbon 1.0g·L -1 Protocorm-like culture induced on the culture medium;

[0026] Solid proliferation of protocorms of Cymbidium: select protocorms with similar growth status, weigh them under sterile conditions, inoculate to 1 / 2MS and add NAA2.0mg L -1 +6-BA1.0mg·L -1 , after 30 days of growth, carry out solid culture proliferation;

[0027] Choose a conical flask with a volume of 100ml as the culture container, and inoculate the protocorm to 1 / 2MS+NAA2.0mg·L -1 +6-BA0.5mg·L -1 On the liquid medium, inoculate 2.0g per bottle;

[0028] Inoculate protocorms to 1 / 2MS+NAA2.0mg·L -1 +6-BA1.0mg·L -1 On the liquid medium, it was cultivated for 20 days, and each bottle was inoculated...

Embodiment 2

[0035] Embodiment two: take the Cymbidium variety '4083' as material to carry out the following operations.

[0036] (1) Test material: Protocorm-like induced stem tip of Cymbidium grandiflora, inoculated to 1 / 2MS+NAA1.0mg·L - 1 +150ml·L -1 Coconut milk + sucrose 30g·L -1 +Agar 6.0g·L -1 +Activated carbon 1.0g·L -1 Protocorm-like culture induced on the culture medium;

[0037] Solid proliferation of protocorms of Cymbidium: select protocorms with similar growth status, weigh them under sterile conditions, inoculate to 1 / 2MS and add NAA2.0mg L -1 +6-BA1.0mg·L -1 , after 30 days of growth, carry out solid culture proliferation;

[0038] Choose a conical flask with a volume of 200ml as the culture container, and inoculate the protocorm to 1 / 2MS+NAA1.5mg·L -1 +6-BA1.0mg·L -1 On liquid medium, inoculate 4.0g per bottle and grow for 20 days;

[0039] Inoculate protocorms to 1 / 2MS+NAA2.0mg·L -1 +6-BA1.0mg·L -1 On the liquid culture medium, carry out 20d culture to it, eac...

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PUM

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Abstract

A manufacturing method for artificial cymbidium seeds includes the following steps of 1 protocorm-like body induction, wherein a cymbidium aseptic seedling stem tip serves as a material, and a protocorm-like body induction medium is inoculated with the cymbidium aseptic seedling stem tip to form a protocorm-like body through induction; 2 protocorm multiplication, wherein after growing in a solid multiplication medium for 20-30 days, the protocorm-like body obtained in the step 1 is transferred to a liquid medium to conduct multiplication and growth for 20-30 days, and the cymbidium protocorm-like body obtained after multiplication is used for manufacturing and embedding the artificial seeds; 3, artificial cymbidium seed manufacturing and germinating, wherein the protocorm-like body obtained in the step 2 is divided into single auxiliary protocorm-like body, a sodium alginate solution and CaCl2 are adopted for embedding, and sterilization and greenhouse germination are conducted on the artificial seeds formed through solidification. The manufacturing method can be used for manufacturing the artificial seeds of different cymbidium varieties, seedlings formed by greenhouse sowing and germinating can replace conventional tissue culture seedlings to produce cymbidium seedlings, and production cost of the cymbidium varieties is reduced.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for making artificial seeds of Cymbidium cymbidium. Background technique [0002] Cymbidium Hybrid (Cymbidium Hybrid) is a hybrid of some large-flowered epiphytic species in the Orchidaceae, and it is one of the highest-selling western orchids in the world. Cymbidium has bright colors, long flowering period and high ornamental value. It is very popular among orchid lovers all over the world. Since Cymbidium is mostly a hybrid variety, the natural seed setting rate of the seeds is quite low, and the seeds are not fully developed and have no endosperm. Under normal circumstances, it is extremely difficult to germinate, and sterile sowing techniques are required to germinate. In addition, the branching ability of Cymbidium is weak, so it is difficult to reproduce, and the propagation speed is slow, which is far from meeting the requirements of commercial pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/006
Inventor 王广东刘昕岑易梦张燕张琛
Owner NANJING AGRICULTURAL UNIVERSITY
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