Method for directly detecting five chemical pollutants in propolis through extractive electrospray ionization mass spectrum

Abstract

The invention belongs to the technical field of detection and discloses a method for directly detecting five chemical pollutants in propolis through extractive electrospray ionization mass spectrum. The method comprises the following steps: 1) preparing a standard substance solution; 2) preparing propolis tincture; 3) preparing marked propolis tincture; 4) performing extractive electrospray ionization mass spectrum detection; 5) performing qualitative analysis; and 6) performing quantitative analysis. According to the detection method provided by the invention, the dosage of the sample is small, the analysis speed is high and the high throughput and on-line detection can be realized; the common propolis chemical pollutants can be directly analyzed and detected, no complex sample pretreatment is required and the detection time for a single sample is less than 1 min. The method is non-toxic and non-pollution to products, operators and working environment. The sensitivity is high. According to the method provided by the invention, the to-be-detected sample is free from complex pretreatment and can bear the influence of complex matrix of propolis; the chemical pollutants in propolis can be directly and quickly detected; and the defects of complex sample pretreatment and long analysis time in the present analysis technique are overcome.

Description

technical field [0001] The invention relates to a method for detecting chemical pollutants in propolis, in particular to a method for directly detecting five kinds of chemical pollutants in propolis by electrospray extraction ionization mass spectrometry. Background technique [0002] Propolis is a resin collected by bees from poplar, betula, willow, oak, horse chestnut and coniferous plants, and mixed with beeswax by bees to form a viscous substance through glandular secretions. Propolis is an effective substance used by bees to maintain the health of the whole group. A colony of 50,000 to 60,000 bees can only produce 70 to 100g of propolis a year, so it is called "purple gold". Due to its diverse biological and pharmacological activities, propolis has properties such as antibacterial, antiviral, antioxidative, anticancer, beautifying and promoting cell regeneration, and is widely used in medicine, cosmetics and food industries. [0003] The residue of chemical pollutants ...

AI Technical Summary

Problems solved by technology

At present, the detection of chemical pollutants in propolis mainly includes high performance liquid chromatography (HPLC), gas chromatography (GC), high performance liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), etc. , they have high sensitivity, but the complex sample pretreatment takes a long time and cannot meet the needs of rapid and high-throughput detection

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