Sample diluent for immunofluorescence chromatography detection

An immunofluorescence and fluorescence immunochromatography technology, which is applied to the field of sample diluent for fluorescence immunochromatography detection, can solve the problems of high cost and complicated process, and achieve the effects of simple operation, overcoming matrix effect and saving material cost.

Inactive Publication Date: 2016-05-25
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is: when the existing fluorescent microsphere antibody is prepared, it is necessary to use a blocking solution containing special components to reduce false positives, the process is complicated and the cost is high, and a kind of sample dilution for fluorescent immunochromatography detection that solves the above problems is provided. liquid

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  • Sample diluent for immunofluorescence chromatography detection
  • Sample diluent for immunofluorescence chromatography detection

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Embodiment

[0019] A sample diluent for fluorescent immunochromatography detection, comprising buffer substances, blocking agents, hydrophilic macromolecular substances, surfactants and sodium chloride.

[0020] Wherein, the buffer substance is TAPS or MOPS, where TAPS is the abbreviation of N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, and MOPS is the abbreviation of 3-morpholinepropanesulfonic acid. The blocking agent is casein. The hydrophilic macromolecular substance is PVA or PVP-K30, the PVA is the abbreviation of polyvinyl alcohol, and the PVP-K30 is the abbreviation of polyvinylpyrrolidone. The surfactant is PluronicF-68 or Brij35.

[0021] Since the buffer substance, the hydrophilic macromolecular substance and the surfactant can be various specific substances, and their action principles are the same, this embodiment only exemplifies one specific substance in each type of substance. The specific settings are shown in Table 1.

[0022] Table 1

[0023]

[0024] ...

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Abstract

The invention discloses a sample diluent for immunofluorescence chromatography detection. The problem that the detection sensitivity and accuracy cannot be more effectively increased when the current pretreatment reagent is only used for preventing the matter from non-specifically adsorbing on an immunochromatography test strip is solved. The sample diluent comprises a buffering matter, a sealing agent, a hydrophilia macromolecule matter, a surface active agent, a metal-chelator, a corrosion remover and sodium chloride. The sample diluent provided by the invention can be used for more effectively increasing the detection sensitivity and accuracy and has an obvious effect.

Description

technical field [0001] The invention relates to a sample diluent, in particular to a sample diluent for fluorescence immunochromatography detection. Background technique [0002] Although the immunochromatographic detection method is simple and fast, there are many types of samples that can be tested, such as serum, plasma, whole blood, urine, etc. Different types of samples have different detection environments during detection, and the samples are complex. The protein components in the test strip will bind to different degrees with the antibodies and fluorescent markers coated on the test strip, which can interfere with the test results, resulting in non-specific and very serious false signals on the test line, that is, false positives. [0003] In the prior art, during the preparation of fluorescent microsphere antibodies, a blocking solution containing special components is used to reduce false positives in biological sample detection, but the preparation process is comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 代敏林源谭韬
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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