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Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling

A technology of southern yew and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, cultivation, etc., to achieve the effects of large-scale production, short hardening time, and convenient transplanting

Active Publication Date: 2016-06-08
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on a quick and easy domestication and transplanting method for in vitro germination of Taxus chinensis tissue seedlings

Method used

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  • Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling
  • Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Selection of tissue culture seedlings: select the root system well-developed, the southern yew tissue culture seedlings of more than 1 true leaf;

[0034] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0035] (3) Preparation of transplanting container and cultivation substrate: select seedling raising box, fill vermiculite in the hole of seedling raising box;

[0036] (4) Transplanting and seedling hardening: implant the tissue cultured seedlings into the middle of the cultivation medium in step (3), pour enough water, close the lid of the seedling raising box, and put it into a greenhouse with 50% sunshade net for cultivation. No more than 35°C, after 2 weeks, the lid of the seedling box can be opened to enter the conventional seedling production management mode, and the survival rate of tissue cultured seedlings transplanted is 100%.

Embodiment 2

[0038] (1) Selection of tissue culture seedlings: select the root system well-developed, the southern yew tissue culture seedlings of more than 1 true leaf;

[0039] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0040] (3) Preparation of transplanting container and cultivation substrate: select a seedling raising box, and fill peat into the holes of the seedling raising box;

[0041] (4) Transplanting and seedling hardening: implant the tissue cultured seedlings into the middle of the cultivation medium in step (3), pour enough water, close the lid of the seedling raising box, and put it into a greenhouse with 50% sunshade net for cultivation. The temperature does not exceed 35°C. After 2 weeks, the lid of the seedling box can be opened to enter the conventional seedling production management mode. The transplanted survival rate of tissue cultured seedlings is 91.67%...

Embodiment 3

[0043] (1) Selection of tissue culture seedlings: select the root system well-developed, the southern yew tissue culture seedlings of more than 1 true leaf;

[0044] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0045](3) The preparation of transplanting container and cultivation medium: select seedling-growing box, fill non-woven fabric and wrap fresh coconut bran powder in the hole of seedling-growing box;

[0046] (4) Transplanting and seedling hardening: implant the tissue cultured seedlings into the middle of the cultivation medium in step (3), pour enough water, close the lid of the seedling raising box, and put it into a greenhouse with 50% sunshade net for cultivation. No more than 35°C, after 2 weeks, the lid of the seedling box can be opened to enter the conventional seedling production management mode, and the survival rate of tissue cultured seedlings tra...

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Abstract

The invention discloses a method for domesticating and transplanting a taxus chinensis var mairei tissue culture seedling. The method comprises the following steps of (1) selecting the tissue culture seedling by means of selecting the taxus chinensis var mairei tissue culture seedling with a well-grown root system and more than one true leaf; (2) preliminarily domesticating the tissue culture seedling by means of putting a tissue culture container with the tissue culture seedling into a greenhouse for 3 to 5 days; (3) preparing a transplanting container and a culture substrate by means of selecting a seedling culturing box, and filling vermiculite, turf or nonwoven-fabric wrapped fresh coconut chaff in holes of the seeding culturing box; (4) transplanting and hardening seedling by means of planting the tissue culture seedling in the middle of the culture substrate obtained in the step (3), watering sufficiently, closing a seedling culturing box cover, putting the box into the greenhouse with 50 percent of sunshield screen, culturing at an environmental temperature of not more than 35 DEG C, and opening the seedling culturing box cover and performing a conventional seedling management mode after two weeks, wherein the survival rate of transplanted tissue culture seedlings reaches 100 percent at most. The method disclosed by the invention has the advantages that the disadvantages in the prior art are overcome, and the effects of simplicity in operation, low cost, high efficiency, stability and reusability of the seedling culturing box are achieved.

Description

technical field [0001] The invention belongs to the field of rapid propagation of yew, and in particular relates to a domestication and transplanting method of tissue cultured seedlings of yew. Background technique [0002] Southern yew (Taxuschinensis var. mairei) is a gymnosperm plant belonging to the genus Taxus of the family Taxaceae, an evergreen tree or shrub, and a dioecious plant. Southern yew is a unique Tertiary relict plant in my country, and it is an important resource plant integrating ornamental, medicinal and timber uses. Since the 1970s, the natural anti-cancer product paclitaxel was extracted and isolated from Taxus brevifolia. Taxus, as an important medicinal plant, has been recognized more and more for its medicinal value. In addition to its anti-tumor pharmacological effects, Taxus chinensis also has various medicinal effects such as anti-inflammatory, anti-bacterial, hepatoprotective, pain-relieving, anti-convulsant, hypoglycemic, anti-oxidative, anti-s...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G9/10
CPCA01G9/029A01G24/00A01H4/00
Inventor 李炎林熊兴耀李莉
Owner HUNAN AGRICULTURAL UNIV
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