Method for detecting bile acid using aptamer-functionalized gold nanoparticles

A gold nanoparticle and bile acid technology, applied in the field of analytical chemistry, can solve the problems of long measurement time, high cost, cumbersome operation, etc., and achieve the effects of low cost, strong visualization, convenient diagnosis and research

Inactive Publication Date: 2016-06-15
CHINA PHARM UNIV
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  • Abstract
  • Description
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Problems solved by technology

Although these methods have high sensitivity, they also have some defects, such as long measurement time, high cost, cumbersome operation, etc.

Method used

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  • Method for detecting bile acid using aptamer-functionalized gold nanoparticles
  • Method for detecting bile acid using aptamer-functionalized gold nanoparticles
  • Method for detecting bile acid using aptamer-functionalized gold nanoparticles

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Embodiment Construction

[0029] The bile acids synthesized by the human liver mainly include cholic acid, chenodeoxycholic acid, deoxycholic acid and a small amount of lithocholic acid and trace ursodeoxycholic acid. Among them, cholic acid is the main component, accounting for about 31%. Therefore, we take the bile acid molecule as an example to describe the present invention in detail. It is necessary to point out that the following examples are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Those skilled in the art Some non-essential improvements and adjustments can be made to the present invention based on the above content of the present invention.

[0030] (1) Preparation of gold nanoparticles

[0031] Material / reagent: chloroauric acid (HAuCl 4 ), trisodium citrate (Na 3 C 6 H 5 O 7 ) Purchased from Sinopharm Chemical Reagent Co., Ltd.

[0032] Method: All glassware is soaked in aqua regia, washed with doubl...

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Abstract

The invention relates to a method for detecting bile acid using aptamer-functionalized gold nanoparticles, and belongs to the technical field of analytic chemistry. The method is characterized by detecting bile acid using the regulating effect of an aptamer on regrowth of gold nanoparticles and comprising the following steps: preparing the gold nanoparticles; preparing a bile acid aptamer-functionalized gold nano probe; checking the affinity between the bile acid and the aptamer through a microcalorimetry analysis technology; detecting a bile acid solution through the bile acid aptamer-functionalized gold nano probe. The method is used for performing in-vitro detection on the bile acid solution and has the advantages of low cost, high visibility, simplicity, quickness and the like; a novel method is provided for content detection of the bile acid and diagnosis of relevant diseases.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for detecting bile acids by using gold nanoparticles functionalized with aptamers. Background technique [0002] Bile acid is an important metabolite of cholesterol decomposition in the liver. It is a general term for a class of 24-carbon cholanoic acid hydroxy derivatives. It helps to emulsify fat, enhance the lipolysis of the pancreas, and can increase fat by forming mixed micelles The solubility of this kind can promote the absorption of lipids in the intestine; it can also mediate the dispersion of cholesterol to form soluble micelles and inhibit the occurrence of gallstones. [0003] The concentration of bile acid in normal human serum is very low, ranging from 4 to 10 μM. Once the metabolism and transport of bile acids are affected, the bile acid levels in human serum, bile and urine will change significantly. Acute and chronic viral hepatitis, bile stasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31
Inventor 顾月清朱秋运马祎
Owner CHINA PHARM UNIV
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