Extraction and separation method of glucosinolate

A technology of glucosinolate and glucoside, which is applied in the field of extraction and separation of glucosinolate preparation, can solve the problems of incomplete deactivation of enzymes, complex desalination process, complicated experiments, etc., achieves good promotion prospects, good purification effect, The effect of high extraction efficiency

Active Publication Date: 2016-06-29
BEIJING UNIV OF CHEM TECH
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Problems solved by technology

It has been verified that using alcohol-containing solvents for extraction has a low extraction rate of glucosinolates and high cost; using acidic alumina or ion exchange resins for purification introduces a large amount of salt, the desalination process is complicated and the recovery rate is low
The Chinese invention patent application with the application number 201210166482.9 discloses a method for extracting glucosinolates from broccoli stem and leaf waste. In this method, after the raw materials are deactivated in a 75°C water bath, 75% methanol aqueous solution is used to extract sulfur for many times. Glucoside substitutes, and then use anion exchange to purify glucosinolates, the enzyme is not completely eliminated, the experiment is complicated, and the cost is high
The Chinese invention patent application with the application number 2015104107303.X discloses a method for extracting sulfur mustard glucosinolates. In this method, the raw materials are dried to inactivate enzymes, grind and degrease with ether, and then use methanol aqueous solution to degrease the raw materials by means of ultrasonic crushing. Glucosinolates are extracted, and ethyl acetate is used for impurity removal and anion exchange resin purification. The purification method is extremely complicated, the energy consumption is high, and the extraction rate is only 65%-75%, and methanol, ether, ethyl acetate, etc. are used toxic reagent

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  • Extraction and separation method of glucosinolate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Raw material pretreatment: crush 200 g of broccoli seeds whose GRA content (mass percentage content, the same below) is 4.01%, to obtain 200 g of broccoli seed powder.

[0025] Glucosinolate extraction: Take 2.3L of deionized water and heat it. After boiling, add the above-mentioned broccoli seed powder to it, extract with boiling water for 10 minutes, and filter through a filter cloth to obtain the GRA water extract. The extraction was repeated once, and the GRA water extract was collected, and the extraction rate of GRA was 98.61%.

[0026] Glucosinolate purification: Concentrate the above-mentioned GRA water extract to 2.3L, add it to 115g of activated carbon, and stir and adsorb at 25°C for 30min. Use an activated carbon filter for solid-liquid separation to obtain GRA-adsorbed activated carbon, add 2.3L of 10% ethanol aqueous solution, adjust the pH to 10.0, and elute at 25°C for 30min, repeat the elution once, and collect the GRA eluate. Spin the above-mentioned ...

Embodiment 2

[0028] Raw material pretreatment: 100 g of broccoli seeds with a GRA content of 6.72% were pulverized to obtain 200 g of broccoli seed powder.

[0029] Glucosinolate extraction: Take 1.9L of deionized water and heat it. After boiling, add the above-mentioned broccoli seed powder to it, extract with boiling water for 10 minutes, and filter through a filter cloth to obtain the GRA water extract. The extraction was repeated once to collect the GRA water extract, and the GRA extraction rate was 98.56%.

[0030] Glucosinolate purification: Concentrate the above GRA water extract to 1.9L, add it to 95g of activated carbon, and stir and adsorb at 25°C for 30min. Use an activated carbon filter for solid-liquid separation to obtain GRA-adsorbed activated carbon, add 1.9L of 10% ethanol aqueous solution, adjust the pH to 10.0, and elute at 25°C for 30min, repeat the elution once, and collect the GRA eluate. The above-mentioned GRA eluent was spin-dried, and 1.9 L of deionized water was...

Embodiment 3

[0032] Raw material pretreatment: 200 g of certain broccoli seeds with a GRA content of 3.47% were pulverized to obtain 200 g of broccoli seed powder.

[0033] Glucosinolate extraction: Take 2.0L of deionized water and heat it. After boiling, add the above-mentioned broccoli seed powder to it, extract with boiling water for 10 minutes, and filter through a filter cloth to obtain the GRA water extract. The extraction was repeated once to collect the GRA water extract, and the GRA extraction rate was 96.98%.

[0034] Glucosinolate purification: Concentrate the above GRA water extract to 2.0L, add it to 100g of activated carbon, and stir and adsorb at 25°C for 30min. Use an activated carbon filter for solid-liquid separation to obtain GRA-adsorbed activated carbon, add 2.0L of 10% ethanol aqueous solution, adjust the pH to 10.0, and elute at 25°C for 30min, repeat the elution once, and collect the GRA eluate. Spin the above-mentioned GRA eluent to dryness, add 2.0L of deionized ...

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Abstract

The invention discloses an extraction and separation method of glucosinolate, and relates to extraction and separation of an active substance in the field of biochemical engineering, in particular to an extraction and separation method of glucosinolate prepared from the raw material of crucifer seeds. According to the method, crucifer seeds are smashed, so that the degree of cell disruption is increased, and glucosinolate is helped to be fully dissolved out; a boiling water extraction method is adopted to extract glucosinolate, and thus the extraction ratio can reach 96.0% or above; an activated carbon adsorption and elution method is adopted to purify glucosinolate, and thus the purity of the obtained glucosinolate product can be increased by 2.5-3.0 times than that of water extract. Compared with a traditional extraction and separation method of glucosinolate, the method is high in glucosinolate extraction efficiency, good in purification effect, simple in process, environmentally friendly, applicable to production and processing of food-grade or health-food-grade glucosinolate and suitable for large-scale industrial production and has good popularization prospects.

Description

technical field [0001] The invention relates to the extraction and separation of effective substances in the field of biochemical industry, in particular to an extraction and separation method for preparing glucosinolates from cruciferous plant seeds. Background technique [0002] Cruciferous plants are a class of plants that are widely believed to have anti-cancer functions. This is mainly because cruciferous plants contain a large amount of glucosinolates (glucosinolates, referred to as GLS), glucosinolates are a sulfur-containing secondary metabolite of plants, and more than 120 kinds of sulfur have been found in plants. Glucosides. Glucosinolates are water-soluble, non-volatile, heat-stable ionic compounds. If plant cells are damaged, glucosinolate glucohydrolase (myrosinase, thioglucosideglucohydrolase; EC3.2.3.1) is released and hydrolyzes glucosinolates to generate bisulfate, glucose and a A series of aglycones, these ligands undergo intramolecular rearrangement to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/14C07H1/08
CPCC07H1/08C07H15/14
Inventor 袁其朋刘玉婷程立王澳克赵妍
Owner BEIJING UNIV OF CHEM TECH
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