Indirect ELISA kit based on Mo P113 protein and use method

Abstract

The invention discloses an indirect ELISA kit based on Mo P113 protein and a use method. The indirect ELISA kit based on Mo P113 protein is prepared from an elisa plate coated with Mo P113 protein, Mo standard positive serum, Mo standard negative serum, bethyl, a 50*PBST buffer solution, a substrate solution A, a substrate solution B and a stop solution. The indirect ELISA kit can detect sheep serum Mo infection antibodies and vaccine immunity antibodies, a key technology is provided for earlier monitoring of goat and sheep mycoplasmal pneumonia caused by Mo, vaccine immunity effect evaluation and correlational research work, and great significance is achieved in early finding and early prevention and control of epidemic diseases caused by the pathogeny.

Description

technical field [0001] The invention relates to an indirect ELISA kit based on MoP113 protein and its application method. Background technique [0002] Mycoplasma ovis pneumoniae is one of the main pathogenic bacteria that cause chronic respiratory infectious diseases in goats and sheep and affect the development of sheep industry. It often harms lambs aged 1 to 3 months. Symptoms of chronic atypical pneumonia such as emaciation, growth retardation, and interstitial pneumonia (EnginBalikci, 2008). In 1963, Mackay et al. isolated Mycoplasma ovis pneumoniae from sheep for the first time, and then Cottew isolated the same pathogenic bacteria from Australian sheep suffering from pneumonia. , 1963; GS. Cottew, 971; Carmichael LE, 1972). In 1978, the pathogen was first isolated from breeding sheep introduced from New Zealand and other places in my country. Since then, the disease has been reported in Guizhou, Yunnan, Gansu, Hunan and other provinces and cities in China (Hu Jings...

AI Technical Summary

Problems solved by technology

The early mycoplasma pneumonia cases caused by Mycoplasma pneumoniae in sheep have a high prevalence and low mortality rate, and have not attracted people's attention for a long time. However, in recent years, investigations have found that the mortality rate of the disease is on the rise, which has brought serious economic losses to the sheep industry. Loss (Zhang Shuangxiang, 2013)

[0003] The prevention and control of Mycoplasma pneumoniae in sheep currently mainly relies on drug prevention, and there is still a lack of reliable vaccines. The reason is related to the time-consuming and laborious isolation and cultivation of Mo and the slow genome research (Hou Xiangshan et al., 2006; Zhao Ping et al., 2008)

The application of clinical vaccines (inactivated sheep infectious pleuropneumonia vaccine) also showed that the vaccine immune control effect on Mo infection epidemic cases was not good, which brought serious economic losses to the sheep industry (Zhang Shuangxiang, 2012)

Moreover, there is currently a lack of rapid serological detection methods required for disease detection and prevention and control, especially the ELISA method with high throughput and high sensitivity

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