Preparation method of biological veterinary drug for preventing and treating hydropericardium hepatitis syndrome in chickens

A technology for pericardial effusion and syndrome, which is applied in the field of preparation of biological veterinary drugs for the prevention and treatment of chicken pericardial effusion-hepatitis syndrome, can solve the problems of high cost and poor effect, and achieve the effects of low cost, high survival rate and long validity period

Inactive Publication Date: 2016-07-20
YANTAI JINHAI PHARMA
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  • Claims
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Problems solved by technology

[0005] In order to overcome the deficiencies in the existing biological veterinary drug technology for preventing and treating chicken pericardial effusion-hepatitis syndrome, the invention provides a preparation method of biological veterinary medicine for preventing and treating chicken pericardial effusion-hepatitis syndrome, solving the problem that there is no suitable treatment for chicken pericardium at present The problem of high cost and poor effect of targeted veterinary drugs for effusion-hepatitis syndrome

Method used

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  • Preparation method of biological veterinary drug for preventing and treating hydropericardium hepatitis syndrome in chickens
  • Preparation method of biological veterinary drug for preventing and treating hydropericardium hepatitis syndrome in chickens

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Embodiment 1

[0050] The preparation steps of the preparation method of the biological veterinary medicine for preventing and treating chicken pericardial effusion-hepatitis syndrome are:

[0051] 1. Antigen

[0052] The antigen used to manufacture the biological veterinary medicine of chicken pericardial effusion-hepatitis syndrome is the allantoic fluid poisonous obtained after inoculating chicken embryos with liver tissue homogenate of pericardial effusion-hepatitis syndrome chicken.

[0053] 2. Manufacture and inspection of antibody IgY for treating chicken pericardial effusion-hepatitis syndrome

[0054] 2.1: Antigen preparation

[0055] 2.1.1 Wash the cryopreserved liver 2 to 3 times with normal saline after thawing to remove the blood and remove the fat from the connective tissue on the surface. Cut it into pieces, wash it repeatedly with normal saline until it becomes bloodless, then add cold normal saline with a weight ratio of 1:5, grind it up and down with a tissue grinder at 1...

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Abstract

The invention relates to the technical field of a biological veterinary drug, in particular to a preparation method of a biological veterinary drug for preventing and treating hydropericardium hepatitis syndrome in chickens. The preparation method comprises steps of preparing an antigen, determining an antigen EID50, preparing an inactivated vaccine, immunizing laying chickens, collecting eggs from hype-immunized chickens and extracting an anti-hydropericardium hepatitis syndrome egg yolk antibody. According to the egg yolk antibody biological veterinary drug for treating the hydropericardium hepatitis syndrome in chickens provided by the invention, chicken embryo proliferating viruses are inoculated to liver tissue homogenate of chickens affected by the hydropericardium hepatitis syndrome in chickens, the laying chickens are immunized by the prepared inactivated vaccine, protein is extracted from egg yolks and the protein is used for preventing and treating the hydropericardium hepatitis syndrome in chickens, so as to solve the problem that a targeted veterinary drug for treating the hydropericardium hepatitis syndrome in chickens is in lack at present and the problems that a current popular treatment scheme is high in cost and poor in effect.

Description

technical field [0001] The invention relates to the technical field of biological veterinary drugs, in particular to a preparation method of biological veterinary drugs for preventing and treating chicken pericardial effusion-hepatitis syndrome. Background technique [0002] Hydropericardium hepatitis syndrome (Hydropericardium hepatitis syndrome, chicken pericardium-hepatitis syndrome), the typical symptom is a sudden onset of broiler chickens aged 3-6 weeks, accompanied by typical symptoms of pericardial effusion and hepatitis, which can also be seen in meat species Chickens and layers. According to research, Fowladenovirus serotype 4 (FAV4) is one of the pathogens causing this disease. It was found that there were basophilic intranuclear inclusion bodies in the liver cells of sick chickens, and after the liver homogenate was aseptically processed, The same condition can be reproduced. The icosahedral virus was observed from the diseased chicken liver under the electron ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61P9/00A61P7/10A61P1/16A61P31/20C07K16/08
CPCC07K16/081A61K2039/505C07K2317/11
Inventor 赵成新莫小见
Owner YANTAI JINHAI PHARMA
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