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Compound protease as well as production method and applications thereof

A compound protease and production method technology, applied in the field of compound protease, production method and application of compound protease, can solve problems such as difficult cross-linking disulfide bond opening, protein utilization barrier barriers, etc., to improve protein utilization, Effects of improving stability and increasing usage

Inactive Publication Date: 2016-07-20
JINANBESTZYME BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current conventional proteases, such as trypsin, pepsin, papain, etc., are difficult to open the cross-linked disulfide bonds in feed protein raw materials, which leads to barriers to the improvement of protein utilization.

Method used

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  • Compound protease as well as production method and applications thereof
  • Compound protease as well as production method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0064] Scheme 1: Application of compound protease on weaned piglets

[0065] Adjust the structure of formula protein raw materials, increase the amount of soybean meal and expanded soybean powder, and reduce the amount of high-quality protein raw materials (such as fish meal, intestinal membrane protein, serum protein powder, etc.)

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Abstract

The invention belongs to the technical field of enzyme preparations, and in particular relates to a compound protease as well as a production method and applications of the compound protease. The compound protease is prepared from the following components in parts by weight: 1-7 parts of alkaline protease, 2-5 parts of acid protease, and 1-4 parts of neutral protease. The gene engineering technology is adopted in the project, the improved bacillus licheniformis containing alkaline protease genes is obtained, the step-by-step scale-up fermentation test is carried out, the fermentation and the aftertreatment technologies are optimized, the preparation improvement is developed, and the follow-up application and development are carried out.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparations, specifically relates to a composite protease, and also relates to a production method and application of the above composite protease. Background technique [0002] Protein feed, energy feed and mineral-vitamin supplement are the three important ingredients of the feed, and they account for a relatively large proportion in the full-price compound feed, generally accounting for 20%-35%. However, the entire feed industry is facing the current situation of shortage of protein feed raw materials and high prices. With the adjustment of the agricultural economic structure, barn breeding has become the main component of farmers' economic income. The scale of breeding is getting larger and larger, and the amount of feed is increasing accordingly. The contradiction of protein feed shortage is becoming increasingly prominent. Ways to improve this situation: On the one hand, it is to find new p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/56C12N9/62A23K20/189A23K50/30A23K50/75C12R1/10C12R1/125C12R1/685
CPCC12N9/54C12N9/62
Inventor 宋迪刘延杰郭方明郭芳坤
Owner JINANBESTZYME BIO ENG CO LTD
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