Blood ammonia determination reagent and preparation method thereof

Abstract

The invention provides a blood ammonia determination reagent. The blood ammonia determination reagent comprises a buffer solution, a preservative and a stabilizing agent, and further comprises leucine dehydrogenase, alpha-ketonic acid NADH (Reduced Form of Nicotinamide-adenine Dinucleotid), beta-hydroxybutyric acid and beta-hydroxybutyrate dehydrogenase, wherein the enzyme activity unit ratio of beta-hydroxybutyrate dehydrogenase to leucine dehydrogenas is 1:10-2,000. According to the blood ammonia determination reagent provided by the invention, reduced coenzyme I(NADH) is oxidized into oxidized coenzyme I (NAD+) by applying a leucine dehydrogenase reaction system; the content of blood ammonia is directly reflected by determining the decrease rate of the reduced coenzyme I in the dominant wave of 340 nm. The sensitivity of the blood ammonia determination reagent can be ensured, and the stability of the NADH can also be ensured by adjusting the proportion of the beta-hydroxybutyrate dehydrogenase to the leucine dehydrogenase to be 1:10 to 2,000 through an experiment.

Description

technical field [0001] The invention relates to the field of reagents for detecting ammonia content, in particular to a kit for measuring blood ammonia by enzymatic method and a preparation method thereof. Background technique [0002] Ammonia is a harmful substance to the human body, and the liver is its main metabolic site. A small amount of free ammonia exists in the blood of healthy people. The source of ammonia in the blood is mainly amino acids produced during protein metabolism in the body, endogenous ammonia decomposed by deamination; the other source is exogenous ammonia decomposed by bacteria in the intestinal tract of protein foods. Under normal circumstances, the liver can synthesize urea from ammonia through the special enzyme system of the ornithine cycle, ornithine carbamoyltransferase, carbamoyl phosphate synthetase, etc., and then excreted through the kidneys. In addition, ammonia in the brain and kidneys reacts with glutamic acid to generate glutamine, whi...

AI Technical Summary

Problems solved by technology

Most blood ammonia kits used in clinical diagnosis are glutamic acid dehydrogenase method, and there are various liquid dosage forms and dry tablet preparations such as single, double, and triple reagents. The main difficulty of the kits is the stability of the reagents

Images