A kind of 6-phosphate glucose dehydrogenase and its coding gene and application

A technology of glucose phosphate and dehydrogenase, which is applied in the fields of application, genetic engineering, and plant gene improvement, and can solve the problems of lack of research on G6PD

Inactive Publication Date: 2019-07-26
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far G6PD has been lack of research in plants and algae

Method used

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  • A kind of 6-phosphate glucose dehydrogenase and its coding gene and application
  • A kind of 6-phosphate glucose dehydrogenase and its coding gene and application
  • A kind of 6-phosphate glucose dehydrogenase and its coding gene and application

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Effect test

Embodiment 1

[0038] 1. Cloning and homology analysis of Nannochloropsis G6PD gene.

[0039] 1. Nannochloropsis oceanica CCAP 849 / 10 (formerly CCMP1779) (purchased from the American Center for Marine Algae and Microorganisms) and Chlorella pyrenoidosa (provided by the Freshwater Algae Species Bank of the Chinese Academy of Sciences) were used as test materials. Nannochloropsis was cultured with liquid f / 2 medium, and the f / 2 medium was pre-filtered with a 0.22 μm filter membrane (purchased from Millipore, Billerica, MA, USA), and the conical culture flask used was filtered through 1.034× 10 5 Pa (121°C) high-pressure steam sterilization for 30 minutes; Nannochloropsis was cultured in an intelligent biological artificial climate incubator, the temperature was 25±1°C, and the light intensity was 200 μmol photons m –2 ·s –1 , The light-to-dark ratio is 15h / 9h. Chlorella pyrenoidosa was cultured with BG11 freshwater medium, and also cultured in an intelligent bioartificial climate incubator ...

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Abstract

The invention discloses G6PD (glucose-6-phosphate dehydrogenase) as well as a coding gene and an application thereof. A base sequence of a G6PD gene is shown as SEQ ID NO.1, and an amino acid sequence of G6PD protein coded by the gene is shown as SEQ ID NO.2. The G6PD gene is obtained from Nannochloropsis oceanica CCAP849 / 10 through cloning for the first time, transformation experiments prove that the gene can affect plant lipid metabolism, promote fatty acid synthesis and increase lipid content. Therefore, the G6PD gene obtained from Nannochloropsis oceanica improves a microalga oil-producing process for genetic engineering, an effective technological means is provided for provision of a microalga strain with excellent performance, and the G6PD has broad application prospect and great economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a glucose-6-phosphate dehydrogenase and its coding gene and application. Background technique [0002] Glucose 6-phosphate dehydrogenase (G6PD) is the key rate-limiting enzyme in the pentose phosphate pathway, which can catalyze the dehydrogenation of glucose 6 phosphate, generate a large amount of NADPH, and provide reducing agents (forces) for various synthetic reactions of cells, such as Involved in the synthesis of fatty acids and sterols. [0003] Studies in recent years have confirmed that the reduction of glucose-6-phosphate dehydrogenase activity during oil accumulation is directly related to the degree of oil accumulation. Therefore, it is of great significance to study the regulation of glucose-6-phosphate dehydrogenase in oleaginous algae to improve the oil content in oleaginous organisms. [0004] In recent years, glucose 6-phosphate dehydrogenase (G6PD) has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/04C12N15/53C12N15/79C12N1/13C12P7/64C12R1/89
CPCC12N9/0006C12P7/6409C12Y101/01049
Inventor 李宏业薛姣杨维东刘洁生
Owner JINAN UNIVERSITY
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