A kind of 6-phosphate glucose dehydrogenase and its coding gene and application
A technology of glucose phosphate and dehydrogenase, which is applied in the fields of application, genetic engineering, and plant gene improvement, and can solve the problems of lack of research on G6PD
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[0038] 1. Cloning and homology analysis of Nannochloropsis G6PD gene.
[0039] 1. Nannochloropsis oceanica CCAP 849 / 10 (formerly CCMP1779) (purchased from the American Center for Marine Algae and Microorganisms) and Chlorella pyrenoidosa (provided by the Freshwater Algae Species Bank of the Chinese Academy of Sciences) were used as test materials. Nannochloropsis was cultured with liquid f / 2 medium, and the f / 2 medium was pre-filtered with a 0.22 μm filter membrane (purchased from Millipore, Billerica, MA, USA), and the conical culture flask used was filtered through 1.034× 10 5 Pa (121°C) high-pressure steam sterilization for 30 minutes; Nannochloropsis was cultured in an intelligent biological artificial climate incubator, the temperature was 25±1°C, and the light intensity was 200 μmol photons m –2 ·s –1 , The light-to-dark ratio is 15h / 9h. Chlorella pyrenoidosa was cultured with BG11 freshwater medium, and also cultured in an intelligent bioartificial climate incubator ...
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