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Biopesticide for ginseng seed and soil treatment and preparation method thereof

A soil treatment and biological pesticide technology, applied in the fields of chemicals for biological control, botanical equipment and methods, preservation of human or animal bodies, etc. The occurrence of diseases and other problems, to achieve the effect of reducing chemical pesticide residues, enhancing resistance, and improving resistance

Active Publication Date: 2016-08-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem is: in the process of planting ginseng, the seedlings and roots are very prone to diseases, such as ginseng damping-off, ginseng blight and ginseng rust, etc. Long-term and large-scale use of chemical pesticides has caused pathogenic bacteria to develop resistance , Chemical pesticide residues in ginseng exceed the standard, and it is difficult to produce high-quality ginseng

Method used

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  • Biopesticide for ginseng seed and soil treatment and preparation method thereof
  • Biopesticide for ginseng seed and soil treatment and preparation method thereof
  • Biopesticide for ginseng seed and soil treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] It is made of the following raw materials in parts by weight: 65 parts of Bacillus amyloliquefaciens freeze-dried powder, 8 parts of auxiliary agent, 1.5 parts of stabilizer, 0.05 part of ultraviolet protective agent, and 10 parts of carrier;

[0022] The bacillus amyloliquefaciens freeze-dried powder is prepared by the following method:

[0023] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number CGMCCNo.9538 in the liquid culture medium, and vibrate at 180r / min in a shaker for 24 hours at 28°C to obtain the seed liquid;

[0024] (2), the seed liquid is inoculated in the seed tank with an inoculation amount of 6% by volume, and the cultivation conditions of the seed tank are: aeration ratio 1:0.9, stirring speed 180r / min, temperature 28°C, tank Pressure 0.015MPa, cultivate until the live bacteria content is 4.5×10 10 CFU / ml, the bacterial suspension was prepared;

[0025] (3) After adding a cryoprotectant to the bacterial suspension, place i...

Embodiment 2

[0033] It is made of the following raw materials in parts by weight: 70 parts of Bacillus amyloliquefaciens freeze-dried powder, 10 parts of auxiliary agent, 2.0 parts of stabilizer, 0.10 part of ultraviolet protective agent, and 17.5 parts of carrier;

[0034] The bacillus amyloliquefaciens freeze-dried powder is prepared by the following method:

[0035] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number CGMCCNo.9538 in the liquid culture medium, and vibrate at 180r / min in a shaker for 24 hours at 28°C to obtain the seed liquid;

[0036] (2), the seed liquid is inoculated in the seed tank with an inoculation amount of 6% by volume, and the cultivation conditions of the seed tank are: aeration ratio 1:1.0, stirring speed 180r / min, temperature 28°C, tank Pressure 0.018MPa, cultivate until the live bacteria content is 4.5×10 10 CFU / ml, the bacterial suspension was prepared;

[0037] (3) After adding a cryoprotectant to the bacterial suspension, plac...

Embodiment 3

[0045] It is made of the following raw materials in parts by weight: 75 parts of Bacillus amyloliquefaciens freeze-dried powder, 12 parts of auxiliary agent, 2.5 parts of stabilizer, 0.15 part of ultraviolet protective agent, and 25 parts of carrier;

[0046] The bacillus amyloliquefaciens freeze-dried powder is prepared by the following method:

[0047] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number CGMCCNo.9538 in the liquid culture medium, and vibrate at 180r / min in a shaker for 24 hours at 28°C to obtain the seed liquid;

[0048] (2), the seed liquid is inoculated in the seed tank with an inoculation amount of 6% by volume percentage, and the cultivation conditions of the seed tank are: aeration ratio 1:1.1, stirring speed 180r / min, temperature 28°C, tank Pressure 0.020MPa, cultivate until the live bacteria content is 4.5×10 10 CFU / ml, the bacterial suspension was prepared;

[0049] (3) After adding a cryoprotectant to the bacterial suspens...

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Abstract

The invention relates to a biopesticide for ginseng seed and soil treatment and a preparation method thereof, and belongs to the field of plant disease biological control. The biopesticide is prepared from, by weight, 65-75 parts of bacillus amyloliquefaciens freeze-dried powder, 8-12 parts of auxiliaries, 1.5-2.5 parts of a stabilizer, 0.05-0.15 part of an ultraviolet protector and 10-25 parts of a carrier. The biopesticide has the strong antibiotic action on various ginseng seedling disease and root disease pathogenic bacteria, not only can improve ginseng seedling resistance, but also effectively decreases the application amount of chemical pesticide, and reduces chemical pesticide residues in ginseng. No organic solvent is adopted in production, pollution in the production process and pollution in products are lowered, no solvent poisoning is generated, no harm is caused to human health, no environmental pollution is caused, and the biopesticide is environmentally friendly.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases, and in particular relates to a ginseng seed, a biological pesticide for planting and soil treatment and a preparation method thereof. Background technique [0002] Ginseng (Panaxginseng) has been approved as a new resource food by the Ministry of Health in 2012, and its consumption and market demand have increased dramatically. Ginseng planting has high requirements on soil and environmental conditions, and is extremely susceptible to infection by various pathogenic bacteria. The main problem is: in the process of planting ginseng, the seedlings and roots are prone to diseases, such as ginseng damping-off, ginseng blight and ginseng rust, etc. Long-term and large-scale use of chemical pesticides has caused pathogenic bacteria to develop resistance 1. Chemical pesticide residues in ginseng exceed the standard, and it is difficult to produce high-quality ginseng. Therefore, it ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P1/00A01P21/00A01G13/00
CPCA01G13/00A01N25/14A01N63/10A01N2300/00
Inventor 高洁左冰刘丽萍王春伟卢宝慧王雪杨丽娜
Owner JILIN AGRICULTURAL UNIV
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