Biopesticide for ginseng seed and soil treatment and preparation method thereof

A soil treatment and biological pesticide technology, applied in the fields of chemicals for biological control, botanical equipment and methods, preservation of human or animal bodies, etc. The occurrence of diseases and other problems, to achieve the effect of reducing chemical pesticide residues, enhancing resistance, and improving resistance

Active Publication Date: 2016-08-03
JILIN AGRICULTURAL UNIV
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem is: in the process of planting ginseng, the seedlings and roots are very prone to diseases, such as ginseng damping-off, ginseng blight and ginseng rust, etc. Long-term and large-sc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biopesticide for ginseng seed and soil treatment and preparation method thereof
  • Biopesticide for ginseng seed and soil treatment and preparation method thereof
  • Biopesticide for ginseng seed and soil treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1

[0021] It is made from the following raw materials in parts by weight: 65 parts of Bacillus amyloliquefaciens freeze-dried powder, 8 parts of auxiliary agents, 1.5 parts of stabilizers, 0.05 parts of UV protectants, and 10 parts of carriers;

[0022] The freeze-dried Bacillus amyloliquefaciens powder is prepared by the following method:

[0023] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number of CGMCC No. 9538 in a liquid medium, and culture it with shaking at 180 r / min in a shaker for 24 hours at 28° C. to prepare a seed liquid;

[0024] (2) Inoculate the seed liquid in a seed tank with an inoculum volume of 6% by volume. The culture conditions of the seed tank are: aeration ratio 1:0.9, stirring speed 180r / min, temperature 28°C, tank Pressure 0.015MPa, cultivate to a live bacteria content of 4.5×10 10 CFU / ml to prepare bacterial suspension;

[0025] (3) Add a cryoprotectant to the bacterial suspension and place it in an ultra-low tempe...

Example Embodiment

[0032] Example 2

[0033] It is made from the following parts by weight of raw materials: 70 parts of Bacillus amyloliquefaciens freeze-dried powder, 10 parts of auxiliary agents, 2.0 parts of stabilizers, 0.10 parts of ultraviolet protectant, and 17.5 parts of carrier;

[0034] The freeze-dried Bacillus amyloliquefaciens powder is prepared by the following method:

[0035] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number of CGMCC No. 9538 in a liquid medium, and culture it with shaking at 180 r / min in a shaker for 24 hours at 28° C. to prepare a seed liquid;

[0036] (2) Inoculate the seed liquid in a seed tank with an inoculum volume of 6% by volume. The culture conditions of the seed tank are: aeration ratio of 1:1.0, stirring speed of 180r / min, temperature of 28°C, and tank Pressure 0.018MPa, cultivate to a live bacteria content of 4.5×10 10 CFU / ml to prepare bacterial suspension;

[0037] (3) Add a cryoprotectant to the bacterial suspension and place i...

Example Embodiment

[0044] Example 3

[0045] It is made of the following raw materials in parts by weight: 75 parts of Bacillus amyloliquefaciens freeze-dried powder, 12 parts of auxiliary agents, 2.5 parts of stabilizers, 0.15 parts of ultraviolet protective agent, and 25 parts of carrier;

[0046] The freeze-dried Bacillus amyloliquefaciens powder is prepared by the following method:

[0047] (1) Inoculate the Bacillus amyloliquefaciens FS6 with the preservation number of CGMCC No. 9538 in a liquid medium, and culture it with shaking at 180 r / min in a shaker for 24 hours at 28° C. to prepare a seed liquid;

[0048] (2) Inoculate the seed solution in a seed tank with an inoculum of 6% by volume. The culture conditions of the seed tank are: aeration ratio of 1:1.1, stirring speed 180r / min, temperature 28°C, tank Pressure 0.020MPa, cultivate to a live bacteria content of 4.5×10 10 CFU / ml to prepare bacterial suspension;

[0049] (3) Add a cryoprotectant to the bacterial suspension and place it in an ultra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Meshaaaaaaaaaa
Login to view more

Abstract

The invention relates to a biopesticide for ginseng seed and soil treatment and a preparation method thereof, and belongs to the field of plant disease biological control. The biopesticide is prepared from, by weight, 65-75 parts of bacillus amyloliquefaciens freeze-dried powder, 8-12 parts of auxiliaries, 1.5-2.5 parts of a stabilizer, 0.05-0.15 part of an ultraviolet protector and 10-25 parts of a carrier. The biopesticide has the strong antibiotic action on various ginseng seedling disease and root disease pathogenic bacteria, not only can improve ginseng seedling resistance, but also effectively decreases the application amount of chemical pesticide, and reduces chemical pesticide residues in ginseng. No organic solvent is adopted in production, pollution in the production process and pollution in products are lowered, no solvent poisoning is generated, no harm is caused to human health, no environmental pollution is caused, and the biopesticide is environmentally friendly.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases, and in particular relates to a ginseng seed, a biological pesticide for planting and soil treatment and a preparation method thereof. Background technique [0002] Ginseng (Panaxginseng) has been approved as a new resource food by the Ministry of Health in 2012, and its consumption and market demand have increased dramatically. Ginseng planting has high requirements on soil and environmental conditions, and is extremely susceptible to infection by various pathogenic bacteria. The main problem is: in the process of planting ginseng, the seedlings and roots are prone to diseases, such as ginseng damping-off, ginseng blight and ginseng rust, etc. Long-term and large-scale use of chemical pesticides has caused pathogenic bacteria to develop resistance 1. Chemical pesticide residues in ginseng exceed the standard, and it is difficult to produce high-quality ginseng. Therefore, it ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/02A01N25/14A01P1/00A01P21/00A01G13/00
CPCA01G13/00A01N25/14A01N63/10A01N2300/00
Inventor 高洁左冰刘丽萍王春伟卢宝慧王雪杨丽娜
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap