The invention discloses printing ink with good color fixation and no penetration. The printing ink is characterized by being prepared from the following raw materials in parts by weight: 20-30 parts of edible alcohol, 1-2 parts of table salt, 0.4-0.8 part of potassium sorbate, 0.02-0.04 part of chamomile essential oil, 3-6 parts of soybean oil, 4-6 parts of castor oil, 7-9 parts of soybean residue, 2-5 parts of castor shells, 3-5 parts of chestnut shells, 1.2-2.5 parts of xanthan gum, 6-8 parts of maltose syrup, 0.03-0.06 part of capsanthin, 13-16 parts of monascus colors and 5-7 parts of additives. The printing ink prepared by adopting the soybean oil, castor oil, soybean residue, castor shells, chestnut shells, monascus colors and other pure natural substances and taking the xanthan gum as a thickening emulsifier has the advantages of good color fixation, no penetration, strong alkali resistance, water resistance, oil resistance, freezing resistance and the like. the printing ink disclosed by the invention can be directly printed on the surfaces of foods and medicines, has bright color, stable performances and good flowability, viscosity and adhesion, is easy to recycle, does not cause great harm to an environment and human bodies, and can meet the color printing needs and meet the eating requirements of a related standard designated by the state.