A kind of yeast water and its preparation method and application in cosmetics
A technology of cosmetics and yeast, applied in the biological field, can solve the problems of less additive dosage, low utilization rate of yeast extraction, bad smell of yeast water, etc., to achieve the effect of highlighting resource utilization and reducing bad smell
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Embodiment 1
[0062] (1) Take 100 μl of Zygosaccharomyces rouxii (Zygosaccharomyces rouxii, CGMCC AS2.1915, China General Microorganism Culture Collection Management Center) preserved in a glycerol tube at -40°C, and spread it on a 9cm plate. The activation medium used is YPD solid culture cultured in a constant temperature incubator at 30°C for 48 hours until the strains were fully activated. Scrape the cultured yeast on the activation medium of the plate to make a bacterial suspension, inoculate it into the yeast primary Erlenmeyer flask medium (BMGY medium), and cultivate it at 30°C and 200rpm for 24 hours; take the yeast cultured in the primary Erlenmeyer flask Culture solution 10% (v / v), expanded to 10 parts of secondary Erlenmeyer flask culture medium (BMGY medium), 30 ℃, 200rpm cultivated 24h, after closing the bottle, the total volume of seed liquid was 1.08L, and the wet weight of thalline was 6 %; Take a first-level seed tank with a volume of 20L, put it into 14 liters of fermenta...
Embodiment 2
[0067] (1) Take 100 μl of Saccharomyces cerevisiae (Saccharomyces cerevisiae, CGMCC 2.1, China General Microorganism Culture Collection Management Center) preserved in a glycerol tube at -40°C, and spread it on a 9 cm plate. The activated medium used is YPD solid medium, 30 Cultivate in a constant temperature incubator at ℃ for 48 hours until the strain is fully activated; scrape the cultured yeast on the activation medium of the plate to make a bacterial suspension, inoculate it into the first-grade Erlenmeyer flask medium (BMGY medium) for yeast, and set the temperature at 30°C, Cultivate at 200rpm for 24h; take 10% (v / v) of the yeast culture solution cultured in the first-level Erlenmeyer flask, expand it into 10 copies of the second-level Erlenmeyer flask culture medium (BMGY medium), culture at 30°C and 200rpm for 24h, and close the flasks The total volume of the rear seed liquid is 0.53L, and the wet weight of the thalline is 6.2%; take a first-level seed tank with a volu...
Embodiment 3
[0072](1) Take 100 μl of Saccharomyces cerevisiae (Saccharomyces cerevisiae, CGMCC 2.1, China General Microorganism Culture Collection Management Center) preserved in a glycerol tube at -40°C, and spread it on a 9 cm plate. The activated medium used is YPD solid medium, 30 Cultivate in a constant temperature incubator at ℃ for 48 hours until the strain is fully activated; scrape the cultured yeast on the activation medium of the plate to make a bacterial suspension, inoculate it into the first-grade Erlenmeyer flask medium (YPDA medium) for yeasts, and store at 30°C, Cultivate at 200rpm for 24h; take 10% (v / v) of the yeast culture solution cultured in the first-level Erlenmeyer flask, expand it into 10 copies of the second-level Erlenmeyer flask culture medium (YPDA medium), culture at 30°C and 200rpm for 24h, and close the flasks The total volume of the rear seed liquid is 0.75L, and the wet weight of the thalline is 6.1%. Take a first-class seed tank with a volume of 15L, put...
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