Skin wound repairing preparation as well as preparation method and application thereof

A skin wound and preparation technology, applied in the field of skin wound repair preparation and preparation thereof, can solve the problems such as the unsafe hidden danger of hyaluronic acid cross-linked preparation and limit clinical use, achieve high safety and clinical use value, promote wound healing, The effect of absorbing wound fluid

Active Publication Date: 2016-08-03
北京方诣生物医药有限公司
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a skin wound repair preparation and its preparation method and application, so as to solve the problem that the preparation of hyaluronic acid cross-linked preparations in the prior art has great unsafe hidden dangers and limits its clinical use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Skin wound repairing preparation as well as preparation method and application thereof
  • Skin wound repairing preparation as well as preparation method and application thereof
  • Skin wound repairing preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Weigh 40g of medical grade hyaluronic acid with a molecular weight of 100KDa, 10g of 4-dimethylaminopyridine, 30g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and dissolve in 3000g Dimethyl sulfoxide, magnetically stirred at room temperature for 2 hours to obtain a hyaluronic acid solution;

[0027] (2) Dissolve 10 g of medical grade pullulan with a molecular weight of 100KDa in 500 g of dimethyl sulfoxide, and stir evenly to obtain a pullulan solution;

[0028] (3) Slowly add 500 g of the pullulan solution prepared in step (2) to 3000 g of the hyaluronic acid solution prepared in step (1) dropwise at 20 drops / min, react at 60°C for 48 hours, and put the reacted Put the solution into a dialysis bag, dialyze with deionized water for 48 hours, pre-freeze the obtained dialysate at -60°C for 24 hours, and freeze-dry the obtained pre-frozen product at -60°C under a vacuum of 0.1 Pa for 24 hours to prepare Spongy skin wound repair preparation.

Embodiment 2

[0030] (1) Weigh 40g of medical grade hyaluronic acid with a molecular weight of 5KDa, 20g of 4-dimethylaminopyridine, 40g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and dissolve in 3000g Dimethyl sulfoxide, magnetically stirred at room temperature for 2 hours to obtain a hyaluronic acid solution;

[0031] (2) Dissolve 10 g of medical grade pullulan with a molecular weight of 100KDa in 500 g of dimethyl sulfoxide, and stir evenly to obtain a pullulan solution;

[0032](3) Slowly add 500 g of the pullulan solution prepared in step (2) to 3000 g of the hyaluronic acid solution prepared in step (1) dropwise at 20 drops / min, react at 80°C for 4 hours, and put the reacted Put the solution into a dialysis bag, dialyze with deionized water for 48 hours, pre-freeze the obtained dialysate at -60°C for 24 hours, and freeze-dry the obtained pre-frozen product at -60°C under a vacuum of 0.1 Pa for 24 hours to prepare Spongy skin wound repair preparation.

Embodiment 3

[0034] (1) Weigh 40g of medical grade hyaluronic acid with a molecular weight of 1000KDa, 8g of 4-dimethylaminopyridine, 20g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and dissolve in 3000g Dimethyl sulfoxide, magnetically stirred at room temperature for 2 hours to obtain a hyaluronic acid solution;

[0035] (2) Dissolve 10 g of medical grade pullulan with a molecular weight of 100KDa in 500 g of dimethyl sulfoxide, and stir evenly to obtain a pullulan solution;

[0036] (3) Slowly add 500 g of the pullulan solution prepared in step (2) to 3000 g of the hyaluronic acid solution prepared in step (1) dropwise at 20 drops / min, react at 40°C for 48 hours, and put the reacted Put the solution into a dialysis bag, dialyze with deionized water for 48 hours, pre-freeze the obtained dialysate at -60°C for 24 hours, and freeze-dry the obtained pre-frozen product at -60°C under a vacuum of 0.1 Pa for 24 hours to prepare Spongy skin wound repair preparation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a skin wound repairing preparation which is prepared by dissolving hyaluronic acid, 4-dimethylamino pyridine, 1-(3-dimethylamino propyl)-3-ethyl carbodiimide hydrochloride in dimethyl sulfoxide, and performing magnetic stirring at the room temperature so as to obtain a hyaluronic acid solution; mixing pulullan with dimethyl sulfoxide, and uniformly stirring so as to obtain a pulullan solution; slowly dripping the pulullan solution into the hyaluronic acid solution to react for 4-48 hours at 40-80 DEG C, dialyzing the reaction liquid, and performing freeze-drying on the obtained dialysis liquid, thereby obtaining the skin wound repairing preparation. The skin wound repairing preparation disclosed by the invention has multiple effects of isolating bacteria, absorbing effusion of a wound surface, keeping the wound surface wet, promoting the wound surface to heal and the like, moreover is wide in preparation raw material resource, low in cost, gentle in synthesis condition, concise in production route, good in operability, and easy in production quality control, and most importantly has relatively higher security and clinical use values when being compared with a conventional similar product.

Description

technical field [0001] The invention relates to a medical material and its preparation method and application, in particular to a skin wound repair preparation and its preparation method and application. Background technique [0002] Wound is tissue damage caused by normal skin under the action of external factors. Common wounds, wound infections and chronic ulcers make it difficult for the skin to heal. Wound healing is a process of regeneration, repair and reconstruction of damaged tissue, and the whole process goes through the stages of inflammation, proliferation and plasticity. For the stage of wound healing, researchers are committed to finding effective ways to promote wound healing. Wound dressing active dressing can affect the speed and quality of wound healing. Its main function is to isolate the wound, prevent infection, stop bleeding and relieve pain, and provide a favorable environment for skin repair. [0003] Pullulan (Pullulan, PL) is an exopolysaccharide p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08B37/08A61K31/715A61K31/728A61P17/02
CPCA61K31/715A61K31/728C08B37/00C08B37/0072
Inventor 杨文智王树根李海鹰
Owner 北京方诣生物医药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap