A kind of contrast agent and its preparation method

A production method and contrast agent technology, which are applied in the medical field, can solve the problems of increasing the biosafety risk and inspection cost of contrast agents, weak contrast agent biotoxicity, etc., and achieve no toxic side effects, good contrast effects, and good biocompatibility Effect

Active Publication Date: 2019-03-15
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to obtain more comprehensive information, it is often necessary to use multiple contrast agents or composite contrast agents at the same time, which greatly increases the biosafety risk and inspection cost of contrast agents in the human body
[0005] At present, the contrast agents under research mainly include: gold nanoparticles (Au NPs), quantum dots, superparamagnetic iron oxide nanoparticles, near-infrared fluorescent dyes, etc., but these contrast agents still cannot solve the problem of poor contrast performance. and biotoxicity issues

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  • A kind of contrast agent and its preparation method
  • A kind of contrast agent and its preparation method
  • A kind of contrast agent and its preparation method

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Embodiment 1

[0057] The contrast agent provided in this embodiment is mainly made of the following raw materials, in parts by weight: 2 parts of diphenylphosphoryl azide, 8 parts of dipalmitoylphosphatidylcholine, 3 parts of PEGylated phospholipids, and 2.5 parts of cholesterol , 1.5 parts of melanin and 8 parts of perfluoropropane.

[0058] The preparation method of the above-mentioned contrast agent comprises the following steps:

[0059] a. Mix diphenylphosphoryl azide, dipalmitoylphosphatidylcholine, phospholipids and cholesterol to obtain a mixed fat, dissolve the mixed fat in ether, and then use a 35KHz ultrasonic cleaner to act for 30 minutes to obtain an ether lipid solution, Wherein, the mass ratio of mixed fat and ether is 20:6.5;

[0060] b. Dissolve melanin in 0.08mol / L sodium hydroxide solution, then use a 35KHz ultrasonic cleaner to act for 12min, and then use 0.008mol / L hydrochloric acid to adjust the pH of the solution under the action of a dialysis bag with a molecular we...

Embodiment 2

[0065] The contrast agent provided in this embodiment is mainly made of the following raw materials, in parts by weight: 4 parts of diphenylphosphoryl azide, 12 parts of dipalmitoylphosphatidylcholine, 5 parts of PEGylated phospholipids, and 3.5 parts of cholesterol , 2.5 parts of melanin and 15 parts of perfluoropropane.

[0066] The preparation method of the above-mentioned contrast agent comprises the following steps:

[0067] a. Mix diphenylphosphoryl azide, dipalmitoylphosphatidylcholine, phospholipids and cholesterol to obtain a mixed lipid, dissolve the mixed lipid in ether, and then use a 45KHz ultrasonic oscillator to act for 5 minutes to obtain an ether lipid solution, Wherein, the mass ratio of mixed fat and ether is 20:11;

[0068] b. Dissolve melanin in 0.12mol / L potassium hydroxide solution, then use a 45KHz ultrasonic oscillator to act for 8 minutes, and then use 0.012mol / L hydrochloric acid to adjust the pH of the solution under the action of a dialysis bag wi...

Embodiment 3

[0076] The contrast agent provided in this embodiment is mainly made of the following raw materials in parts by weight: 3 parts of diphenylphosphoryl azide, 10 parts of dipalmitoylphosphatidylcholine, 4 parts of PEGylated phospholipids, and 3 parts of cholesterol , 2 parts of melanin and 11 parts of perfluoropropane.

[0077] The preparation method of the above-mentioned contrast agent comprises the following steps:

[0078] a. Mix diphenylphosphoryl azide, dipalmitoylphosphatidylcholine, phospholipids and cholesterol to obtain a mixed lipid, dissolve the mixed lipid in ether, and then use a 40KHz ultrasonic cleaner to act for 20 minutes to obtain an ether lipid solution, Wherein, the mass ratio of mixed fat and ether is 20:7.2;

[0079] b. Dissolve melanin in 0.1mol / L sodium hydroxide solution, then use a 40KHz ultrasonic cleaner to act for 10 minutes, and then use 0.01mol / L hydrochloric acid to adjust the pH of the solution under the action of a dialysis bag with a molecula...

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Abstract

The invention provides a contrast medium and a preparation method thereof and relates to the technical field of medical treatment. The contrast medium is mainly prepared from, by weight, 2-4 parts of diphenyl azidophosphate, 8-12 parts of dipalmitoyl phosphatidyl choline, 3-5 parts of phospholipid, 2.5-3.5 parts of cholesterol, 1.5-2.5 parts of melanin and 8-15 parts of perfluoropropane, wherein the phospholipid refers to PEGylated phospholipid. The contrast medium has the advantages of good biocompatibility, no toxic or side effect and good contrast effect. The preparation method of the contrast medium is simple in steps and convenient to operate.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a contrast agent and a production method thereof. Background technique [0002] Medical imaging plays an important role in the diagnosis of cancer. Imaging techniques currently used in clinical practice include traditional X-ray, ultrasound, computed tomography (CT), and magnetic resonance imaging (MRI), photoacoustic imaging, single photon emission computed tomography (SPECT), and positive imaging developed in recent years. Electron emission tomography (PET), etc. However, single molecular imaging technology has some insurmountable defects, such as limitations in time and space resolution, penetration depth and energy extension. A single imaging method is not enough to fully obtain tumor information, and the combination of multi-modal imaging technologies can complement each other's advantages and provide more accurate and comprehensive information for a clear diagnosis. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K49/00
CPCA61K49/00A61K49/221
Inventor 王冬杨珂姚远志张亮李娅莎钟英郑妍
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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