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Bluetongue antibody competition liquid and corresponding quick ELISA detection method

A detection method and technology for bluetongue, applied in the field of bluetongue virus, can solve problems such as long detection time, achieve the effects of convenient and rapid detection, simplify experimental steps, and shorten diagnosis time

Inactive Publication Date: 2016-08-10
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity and repeatability of the two ELISA methods can meet the detection requirements, but the detection time is longer, of which B-ELISA takes 4 hours and C-ELISA takes 3 hours

Method used

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  • Bluetongue antibody competition liquid and corresponding quick ELISA detection method
  • Bluetongue antibody competition liquid and corresponding quick ELISA detection method

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Embodiment 1

[0034] Example 1, a bluetongue antibody competition solution provided by the present invention, comprising the following raw materials: BTV monoclonal antibody, anti-mouse HPR enzyme-labeled secondary antibody, negative bovine serum, thimerosal, protein protectant, wherein the BTV monoclonal antibody is BTV VP7 monoclonal antibody, the effect and the antibody to be tested compete for the antigenic site, from the monoclonal antibody hybridoma cell line No. Hybasvigl01 (Wuhan China Cell Bank); the anti-mouse HPR enzyme-labeled secondary antibody, the effect and the monoclonal antibody combination, provide HPR enzyme activity, produced by Provided by Invitrogen; negative bovine serum to prevent non-specific reactions, provided by Gibco; thimerosal has anti-corrosion and quality preservation; protein protectant has the effect of protecting proteins, and comes from the product Conjagate stabilizer prod#37548 of Thermo Company.

[0035] Another aspect of the present invention, a prep...

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Abstract

The invention discloses bluetongue antibody competition liquid and a corresponding quick ELISA detection method. The method comprises the steps of obtaining an antigen site based on a BTV antigen curing panel through competition between an antibody to be detected and the antibody competition liquid, and conducting substrate development based on the antigen site. On the premise that the sensitivity and repeatability of original BTV C-ELISA are maintained, the number of experiment steps is reduced to be two, diagnosis time is shortened to be within one hour, and bluetongue serum antibody detection is made more convenient and faster.

Description

technical field [0001] The invention relates to the field of bluetongue virus, in particular to a bluetongue antibody competition solution and a corresponding rapid ELISA detection method. Background technique [0002] Bluetongue (Blue Tongue, BT) is caused by Blue Tongue Virus (Blue Tongue Virus, TV) and is a viral infectious disease of ruminants transmitted by Culicoides. animal disease. Serological detection of bluetongue is an important item of animal import and export inspection. Serological detection refers to the method of distinguishing healthy animals from infected animals by detecting the antibodies produced by bluetongue-infected animals. At present, bluetongue is used internationally Serological detection methods include indirect bluetongue enzyme-linked immunosorbent assay (ELISA) and bluetongue agar diffusion assay (AGID). The AGID method has been gradually replaced by the ELISA method due to its insufficient sensitivity. The basic principle of ELISA is: the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569
CPCG01N33/56983G01N33/577G01N2333/14
Inventor 李乐李华春苗海生廖得芳寇美龄朱建波高林王金平
Owner YUNNAN ANIMAL SCI & VETERINARY INST