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Artificial multiplication method for tetrastichus septentrionalis

The invention relates to a technology for artificial reproduction of the black stick larvae, which is applied in the field of insect reproduction and achieves the effects of increasing the parasitic rate, strengthening the contact of bee pupae, and having a high parasitic rate.

Active Publication Date: 2016-08-17
LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no literature reports on the use of tussah silkworm chrysalis and other records to reproduce the white moth black gnat on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: a kind of artificial breeding method of white moth black stick gnat, comprises the following steps:

[0031] (a) Select an alternative host: use the autumn pupae of Northeast tussah silkworm as an alternative host and store them in a cold storage with the temperature controlled at 4°C and the relative humidity kept at 70%; A warm cocoon warmed up to ambient temperature;

[0032] (b) Field collection and screening of the pupae of Yang Xiaozhou moth: collect the pupae of Yang Xiaozhou moth in nature, and screen the parasitic pupae and unparasitic pupae; body surface bright;

[0033] (c) bee seed cultivation: parasitic pupa is put into test tube culture, and the black rod gnat after eclosion is used as kind of bee or the first generation tussah silkworm chrysalis that is multiplied is used as kind of bee;

[0034] (d) Information substance extraction: Soak the unparasitic pupae of A. japonica with 40% alcohol at a concentration of 20 heads / 100ml for 10 hour...

Embodiment 2

[0038] Embodiment 2: a kind of artificial propagation method of white moth black stick gnat, comprises the following steps:

[0039] (a) Select an alternative host: use the autumn pupae of Northeast tussah silkworm as an alternative host and store them in a cold storage with the temperature controlled at 6°C and the relative humidity kept at 80%; A warm cocoon warmed up to ambient temperature;

[0040] (b) Field collection and screening of the pupae of Yang Xiaozhou moth: collect the pupae of Yang Xiaozhou moth in nature, and screen the parasitic pupae and unparasitic pupae; body surface bright;

[0041] (c) bee seed cultivation: parasitic pupa is put into test tube culture, and the black rod gnat after eclosion is used as kind of bee or the first generation tussah silkworm chrysalis that is multiplied is used as kind of bee;

[0042] (d) Information substance extraction: Soak the unparasitized pupae of A. japonica with 50% alcohol at a concentration of 40 heads / 100ml for 15...

Embodiment 3

[0046] Embodiment 3: a kind of artificial breeding method of white moth black stick gnat, comprises the following steps:

[0047] (a) Select an alternative host: use the autumn pupae of Northeast tussah silkworm as an alternative host and store them in a cold storage with the temperature controlled at 5°C and the relative humidity kept at 75%; A warm cocoon warmed up to ambient temperature;

[0048] (b) Field collection and screening of the pupae of Yang Xiaozhou moth: collect the pupae of Yang Xiaozhou moth in nature, and screen the parasitic pupae and unparasitic pupae; body surface bright;

[0049] (c) bee seed cultivation: parasitic pupa is put into test tube culture, and the black rod gnat after eclosion is used as kind of bee or the first generation tussah silkworm chrysalis that is multiplied is used as kind of bee;

[0050] (d) Information substance extraction: Soak the unparasitic pupae of A. japonica with 45% alcohol at a concentration of 30 heads / 100ml for 13 hour...

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Abstract

The invention discloses an artificial multiplication method for tetrastichus septentrionalis. The method includes the following steps of (1) selection of a substitute host; (2), field collection and selection of micromelalopha troglodyta pupa; (3), cultivation of bee species; (4) extraction of information matter; (5), processing of antheraea pernyi pupa; (6), bee receiving; (7), cultivation and development. According to the artificial multiplication method, pupa which is not subjected to parasitism is soaked with ethyl alcohol, pupa surface extract is obtained, then the pupa surface extract is sprayed on the antheraea pernyi pupa in an artificial spraying mode, the tetrastichus septentrionalis is induced to be subjected to parasitism, and the parasitism rate is raised. The tetrastichus septentrionalis is subjected to indoor large-scale multiplication, and a practical technical means is provided for preventing and controlling hyphantria cunea, micromelalopha troglodyta and other lepidoptera pests for forest creatures.

Description

technical field [0001] The invention relates to the field of insect propagation, in particular to an artificial propagation method of the black-cripped wasp. Background technique [0002] The white moth Tetrastichus septentrionalis Yang is an important parasitic natural enemy in the pupal stage of various Lepidoptera leaf-eating pests such as American white moth, Yang Xiaozhou moth, and Yang fanzhou moth. It is widely distributed in northern my country. A relatively common natural enemy insect in the pupal stage of the order pests. Generally, 86-349 bees can emerge from each pupa of the American white moth, with an average of 160 bees. The wasp adult is relatively large, strong in body, strong in flying ability, and strong in vitality. It can survive for more than 15 days at room temperature. The natural parasitism rate in the pupae of the American white moth is sometimes as high as 12% to 24%. It has great potential in biological control. Good use prospects. [0003] Tussa...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 郭振业刘全山林艳平孙会灵陈慧杰万陈真袁晓菲吕霞王瑞承青春闫俊香白纪霞李国强张峰薛科郭小伟梁晓红翟耀东葛瑞琼杨金泽姬锟李大银王振章张合成伊丽亚任慧娟
Owner LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
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