Method for preparing dendrobium officinale peptide

A technology of dendrobium and candidum officinale, which is applied in the field of preparation of dendrobium peptides, can solve the problems that the separation, purification and application of dendrobium polypeptides have not yet been reported, and achieves the effects of unique, simple and practical preparation method, high absorption rate by human body, and saving drug resources.

Inactive Publication Date: 2016-08-17
JINZHAI JINLIYUAN BIOTECH CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] At present, there are no reports on the separation, purification and application of Dendrobium polypeptide at home and abroad.

Method used

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Embodiment Construction

[0018] In order to make it easy to understand the technical means, creative features, objectives and effects achieved by the present invention, the present invention will be further described with reference to specific embodiments below.

[0019] A preparation method of dendrobium peptide, the specific method steps are as follows:

[0020] (1) Use dried Dendrobium candidum (Fengdou) as a processing raw material, put it in warm water and soak for about 20 minutes, and keep the water temperature at 35-40℃. For every 500g of warm water, add 30g white wine, 20g salt, 15g apple cider vinegar, 30g of ginger juice, 15g of soy protein isolate, 10g of saponin, 8g of onion extract, 5g of psyllium extract, 3g of nepeta extract; using the above method to clean can effectively remove debris and germs on the surface of dendrobium;

[0021] (2) Remove the cleaned dendrobium to drain the surface water, send it to the low-temperature freezing equipment, and treat it at low temperature at -15℃ to -22℃...

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Abstract

The invention discloses a method for preparing dendrobium officinale peptide. The method comprises the following steps: 1) taking dried dendrobium officinale as a processing raw material, and soaking in warm water; 2) fishing out the cleaned dendrobium officinale, draining off the water on the surface, and performing low-temperature treatment in low-temperature freezing equipment; 3) taking out the dendrobium officinale after low-temperature treatment, cutting into dendrobium officinale fragments of 0.5-20mm, adding pure water which is 0.5 times of the weight of the dendrobium officinale, and kneading in a kneading machine together; 4) taking out the kneaded dendrobium officinale fragments, performing superfine crushing, screening by using a medicine sieve of 100-400 meshes, and uniformly mixing with pure water in the kneading machine in the step 3); 5) feeding the wet dendrobium officinale powder into microwave treatment equipment, and performing intermittent microwave treatment at the microwave frequency of 2450MHz and the power of 500W; 6) adding water into the wet dendrobium officinale powder after microwave treatment in a weight ratio of 1:(12-18), soaking for 1.5 hours in a water bath of 75 DEG C, adding papain, and performing extraction for 1-4 hours so as to obtain an extraction liquid; 7) performing vacuum filtration by using a circulating water type multi-purpose vacuum pump so as to obtain a peptide liquid after suction filtration; and 8) performing spray-drying on the peptide liquid, thereby obtaining the dendrobium officinale peptide.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing dendrobium peptides. Background technique [0002] Dendrobium is a perennial herb, also known as black knotweed. Its dried stems can be processed into maple buckets. It is recorded in medical books such as "Tao Zang", "Shen Nong Materia Medica", "Compendium of Materia Medica Supplements" and so on. Known as the first of the nine great immortals in China. The 2012 edition of the "Pharmacopoeia of the People's Republic of China" records that Dendrobium officinale has the effects of nourishing the stomach, nourishing fluid, nourishing yin and clearing heat, and can be used for the symptoms of bone steaming, insufficient stomach yin, yin deficiency and fire, dry mouth and polydipsia. In recent years, with the rapid development of separation technology and biotechnology, researchers have been able to conduct detailed studies on the chemical components and bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 洪先进洪勇
Owner JINZHAI JINLIYUAN BIOTECH CO LTD
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