Method for determining toxicity of pesticide wettable pulvis by using Eisenia fetida

A wettable powder, pure seed technology, applied in the field of aquaculture, can solve the problems of lack of research data to evaluate the practicability and reliability of the model, and achieve the effects of strong practicability, high accuracy and easy operation.

Inactive Publication Date: 2016-08-17
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on the use of Eisenia worm as a pesticide wettable powder toxicity screeni

Method used

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  • Method for determining toxicity of pesticide wettable pulvis by using Eisenia fetida
  • Method for determining toxicity of pesticide wettable pulvis by using Eisenia fetida
  • Method for determining toxicity of pesticide wettable pulvis by using Eisenia fetida

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for determining the toxicity of pesticide wettable powders by earthworms of the present invention includes the following steps:

[0026] 1. Preparation of test solution

[0027] Weigh 6.6667 g of 15% clodinafop-propargyl wettable powder, dissolve it with distilled water and dilute to a 100 mL volumetric flask to obtain a stock solution with a concentration of 10.0 mg a.i. / mL.

[0028] Experimental group: Take 5 parts of 1500g artificial soil (dry weight), pipette 1.500, 3.000, 6.00, 12.00, 24.00mL of the above stock solution into 1500g artificial soil (dry weight) to obtain the mixture (labeled 1 , 2, 3, 4, 5). Add 448, 447, 444, 438, 426 mL of distilled water to the mixtures 1 to 5, and stir thoroughly to mix the liquid, soil and water uniformly to obtain concentrations of 10.0, 20.0, 40.0, 80.0, 160mg a.i. / kg, respectively Dry soil The test solution. Divide each of the above-mentioned test liquids into three equally, as parallel samples, which are respectively co...

Embodiment 2

[0048] A method for determining the toxicity of pesticide wettable powders by earthworms of the present invention includes the following steps:

[0049] 1. Preparation of test solution

[0050] Weigh 0.1000 g of the 50% dinotefuran wettable powder, dissolve it with distilled water and dilute to a 50 mL volumetric flask to obtain a stock solution with a concentration of 1.00 mg a.i. / mL.

[0051] Experimental group: Take 5 parts of 1500g artificial soil (dry weight), pipette 0.150, 0.270, 0.486, 0.874, 1.580 mL of the above stock solution into 1500g artificial soil (dry weight) to obtain the mixture (labeled 1 , 2, 3, 4, 5). Add 395 mL of distilled water to the mixtures 1 to 5, stir well to mix the liquid, soil and water uniformly, and obtain concentrations of 0.100, 0.180, 0.324, 0.583, 1.050 mg a.i. / kg, respectively Dry soil The test solution. Divide each of the above-mentioned test liquids into three equally, as parallel samples, which are respectively contained in 1L specimen bo...

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Abstract

The invention discloses a method for determining the toxicity of pesticide wettable powder by Chizi Aisheng earthworm, which comprises the following steps: dissolving the pesticide wettable powder to prepare a pesticide stock solution; Gradient concentration of the test solution; select Eisenia earthworms with reproductive zones, randomly add them to the biological test solution, continue to cultivate for 14 days, and record the biological effect parameters of Eisenia earthworms on the 7th and 14th days; according to the biological effect parameters of Eisenia earthworms Calculation of the median lethal concentration LC of the pesticide wettable powder on the chrysalis Eisenia earthworm with effect parameters 50 value to judge the toxicity of pesticide wettable powder; it has the advantages of easy operation, strong practicability, fast speed and high accuracy, and can preliminarily judge the toxicity of pesticide wettable powder.

Description

Technical field [0001] The invention relates to the field of breeding technology, and in particular to a method for determining the toxicity of a pesticide wettable powder by earthworms. Background technique [0002] Eisenia foetida belongs to the genus Eisenia foetida of the Annelid oligochaeidae Orthophagidae. The life cycle of Eisenia foetida is short. Under the condition of 20℃, it takes 3 to 4 weeks to incubate, 7 to 8 weeks. Can mature. It has strong reproductive ability. One earthworm can produce 2 to 5 cocoons within a week, and each cocoon can hatch several earthworms. It has the characteristics of high reproduction rate, strong adaptability and suitable for artificial breeding. Therefore, the earthworm has become a commonly used model organism in scientific research and is widely used in life sciences, toxicology, environmental sciences and agricultural sciences. [0003] At present, there are few studies on using Eisenia vulgaris as the toxicity screening model of pes...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 陈昂刘勇张德咏奚庆罗源华罗香文刘志邦严清平何安頔刘建宇
Owner HUNAN PLANT PROTECTION INST
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