Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for Determining the Toxicity of Pesticides and Fungicides by Model Algae

A fungicide and biological technology, applied in the field of aquaculture, can solve the problems of lack of suitability and reliability of research data, and achieve the effects of easy evaluation end point, short test period and strong practicability.

Active Publication Date: 2018-11-06
HUNAN PLANT PROTECTION INST
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few research reports on the toxicity screening of pesticides and fungicides at home and abroad. Even if there are some reports and related research models, there is a lack of relevant research data to evaluate the suitability and reliability of the model, and it has become a mature and recognized toxicity model. screening model

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for Determining the Toxicity of Pesticides and Fungicides by Model Algae
  • Method for Determining the Toxicity of Pesticides and Fungicides by Model Algae
  • Method for Determining the Toxicity of Pesticides and Fungicides by Model Algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for measuring the toxicity of pesticide fungicides by model organism algae of the present invention comprises the following steps:

[0030] S1. Preparation of test liquid:

[0031] Preparation of BG11 medium dilution: Prepare the BG11 medium formula according to the formula in Table 1. The preparation method is: prepare the ingredients in Table 1 to make the corresponding mother solution concentration, and transfer the corresponding mother solution dosage to the 1000mL volumetric flask according to the indicated order and distilled water to 1000mL, autoclaved at 121°C for 15 minutes, sealed and labeled, stored in a refrigerator at 4°C, and valid for 2 months. Dilute the BG11 medium 10 times with distilled water to obtain the BG11 medium dilution.

[0032] Weigh 0.1000 g of 50% difenoconazole suspension, dissolve it with the BG11 medium dilution and dilute it to a 100 mL volumetric flask to obtain a stock solution with a concentration of 500 mg a.i. / L. Pipette...

Embodiment 2

[0054] A method for measuring the toxicity of pesticide fungicides by model organism algae of the present invention comprises the following steps:

[0055] 1. Test liquid preparation:

[0056] Pipette 0.625mL of 80 g / L tebuconazole suspension seed coating agent, dissolve and settle to 100mL volumetric flask with BG11 culture medium dilution (the composition of BG11 dilution is identical with embodiment 1), obtain concentration and be 500mg a.i. / L stock solution. Pipette 0.300, 0.600, 1.200, 2.400, 4.800mL of the above stock solution in sequence, respectively dilute to 250mL volumetric flask with BG11 culture medium dilution, and prepare the concentration of 0.600, 1.20, 2.40, 4.80, 9.60mg a.i. / L 2 times the test liquid.

[0057] S2, the preparation of algae dilution: measure the cell concentration of algae stoste (Cercis cerevisiae) with hemocytometer method and be 4.00 * 10 6 , and diluted 100 times with sterile water to obtain the algae dilution, the concentration of alga...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring the toxicity of a pesticide fungicide by a model biological algae, which comprises the following steps: preparing the pesticide fungicide into a gradient concentration test liquid; diluting the raw liquid of the biological algae with sterile water to prepare an algae dilution; The test liquid is mixed with the algae diluent, cultured under sealed conditions for 72 hours, the algae cells are counted every 24 hours, and the biological effects of the algal cells and the physical and chemical parameters of the test solution are recorded; The half effect concentration EC50 of cells is used to judge the toxicity of pesticides and fungicides. The method of the invention has the advantages of easy operation, strong practicability, short cycle, easy judgment of the evaluation end point and the like.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a method for measuring the toxicity of pesticide fungicides by model algae. Background technique [0002] As the primary producers of aquatic ecosystems, algae play an extremely important role in the balance and stability of water bodies. Algae testing in biological testing is an essential method in aquatic ecotoxicology research. After pesticides are used in the field, they will migrate into the water body and affect the growth of algae, thus affecting the entire ecosystem. Algae are often used in the research of algae growth inhibition toxicity test in the registration of new chemical substances and in the research of algae growth inhibition toxicity test in pesticide registration. [0003] At present, there are few research reports on the toxicity screening of pesticides and fungicides at home and abroad. Even if there are some reports and related research models, there ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 刘勇张德咏罗香文陈源蒋桂芳罗杰陈昂奚庆罗源华刘志邦严清平何安頔刘建宇
Owner HUNAN PLANT PROTECTION INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com