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Method for determining toxicity of pesticide suspending agents by aid of eisenia fetida

A kind of suspending agent and pure seed technology, applied in the field of breeding, can solve the problems of lack of research data to evaluate the practicability and reliability of the model, and achieve the effect of strong practicability, fast speed and easy operation

Inactive Publication Date: 2016-06-08
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on the use of Eisenia worm as a toxicity screening model for pesticide suspensions, and there is a lack of relevant research data to evaluate the practicability and reliability of the model

Method used

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  • Method for determining toxicity of pesticide suspending agents by aid of eisenia fetida
  • Method for determining toxicity of pesticide suspending agents by aid of eisenia fetida
  • Method for determining toxicity of pesticide suspending agents by aid of eisenia fetida

Examples

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Effect test

Embodiment 1

[0024] A kind of method for measuring the toxicity of pesticide suspension concentrates of the present invention, comprising the following steps:

[0025] 1. Test solution preparation

[0026] Weighed 0.8333 g of 30% pyraclostrobin suspension concentrate, dissolved it in distilled water and fixed the volume into a 50 mL volumetric flask to obtain a stock solution with a concentration of 5.00 mga.i. / mL.

[0027] Experimental group: Take 5 parts of 1500g of artificial soil (dry weight), and pipette 2.787, 3.900, 5.46, 7.65, 10.71, 15.00mL of the above stock solution into 1500g of artificial soil (dry weight) with a pipette to obtain a mixture (label as 1, 2, 3, 4, 5). Add 447, 446, 445, 442, 439, and 435 mL of distilled water to mixtures 1 to 5, stir well to mix the liquid medicine, soil, and water evenly, and obtain concentrations of 9.29, 13.0, 18.2, 25.5, 35.7, and 50.0 mga.i. / kg 干土 test solution. Each of the above-mentioned test solutions was equally divided into three p...

Embodiment 2

[0047] A kind of method for measuring the toxicity of pesticide suspension concentrates of the present invention, comprising the following steps:

[0048] 1. Test solution preparation

[0049] Weigh 0.2500 g of 20% dinotefuran suspending agent, dissolve it with distilled water and set the volume to a 50 mL volumetric flask to obtain a stock solution with a concentration of 1.00 mga.i. / mL.

[0050]Experimental group: Take 5 parts of 1500g of artificial soil (dry weight), pipette 0.150, 0.300, 0.600, 1.200, 2.400, 4.800mL of the above stock solution into 1500g of artificial soil (dry weight) with a pipette to obtain a mixture (label as 1, 2, 3, 4, 5). Then add 450, 450, 449, 449, 448, and 445 mL of distilled water to mixtures 1 to 5, stir well to mix the liquid medicine, soil, and water evenly, and obtain concentrations of 0.100, 0.200, 0.400, 0.800, 1.60, and 3.20, respectively. mga.i. / kg 干土 test solution. Each of the above-mentioned test solutions was equally divided into ...

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Abstract

The invention discloses a method for determining the toxicity of pesticide suspending agents by the aid of eisenia fetida. The method includes steps of dissolving the pesticide suspending agents to prepare pesticide reserving solution; adding the pesticide reserving solution into mixtures of soil and water to prepare test solution with gradient concentration; selecting the eisenia fetida with clitella, randomly adding the eisenia fetida into the test solution, continuously cultivating the eisenia fetida for 14 d and recording biological effect parameters of the eisenia fetida on the 7th d and the 14th d; computing medium lethal concentration LC50 values of the pesticide suspending agents for the eisenia fetida according to the biological effect parameters of the eisenia fetida and judging the toxicity of the pesticide suspending agents. The method has the advantages of easiness in operation, high practicality, speed and accuracy and the like. Besides, the toxicity of the pesticide suspending agents can be preliminarily judged by the aid of the method.

Description

technical field [0001] The invention relates to the field of breeding technology, in particular to a method for measuring the toxicity of pesticide suspensions by chrysostasis earthworms. Background technique [0002] Eiseniafoetida is a terrestrial annelid widely distributed in the world, belonging to the genus Eiseniafoetida of the phylum Oligochaeteidae. Chizi Aisheng earthworm can loosen the soil, improve the soil, improve soil fertility, and promote agricultural production. It has a short life cycle. Under the condition of 20°C, it takes 3-4 weeks to hatch, and it can mature in 7-8 weeks. It has strong reproductive ability. One earthworm can produce 2 to 5 earthworm cocoons within a week, and each earthworm cocoon can hatch several earthworms. It has the advantages of high reproduction rate, strong adaptability, suitable for artificial breeding, sensitive to poisons in the environment, and simple test methods, so it has been valued by many domestic and foreign toxicol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 刘勇张德咏陈昂奚庆罗源华罗香文刘志邦严清平何安頔刘建宇
Owner HUNAN PLANT PROTECTION INST
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