Method for evaluating microplastic toxicity based on daphnia magna behavioral indexes

A technology of large daphnia and microplastics, applied in scientific instruments, analytical materials, material inspection products, etc., can solve the problems of inability to achieve rapid evaluation, strict experimental conditions, long experimental cycle, etc., and achieve strong practicability, low cost, and easy operation easy effect

Pending Publication Date: 2021-08-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the toxic effects of Daphnia magna are generally assessed from the cell and tissue levels, and the experimental period is long and the experimental conditions are relatively strict, so rapid evaluation cannot be achieved.

Method used

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  • Method for evaluating microplastic toxicity based on daphnia magna behavioral indexes
  • Method for evaluating microplastic toxicity based on daphnia magna behavioral indexes
  • Method for evaluating microplastic toxicity based on daphnia magna behavioral indexes

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Comparing the biological toxicity of polystyrene microplastics with different particle sizes based on the behavioral indicators of Daphnia magna:

[0033] (1) The selected Daphnia magna had been cultivated continuously for one year under laboratory conditions. Specifically, Daphnia magna was cultured in reconstituted water configured with ultrapure water, and every 1000 mL of reconstituted water contained 58.5 mg of CaCl 2 2H 2 O, 24.7mg MgSO 4 ·7H 2 O, 13.0mg NaHCO 3 and 1.2mg KCl, and aerated for 24 hours before culturing; cultivate Daphnia magna in a light incubator, set the temperature at 22±1°C, and have a light-dark ratio of 16h:8h. Daphnia magna was fed with Scenedesmus obliques every day, and the feeding density was 10 5 cells / mL; Healthy adult Daphnia magna individuals from the same female line, 3 generations of parthenogenesis, and 7 days of age were selected for the exposure test.

[0034] (2) The aqueous suspensions of standard polystyrene microplastic...

Embodiment 2

[0041] The biological toxicity of different concentrations of polystyrene microplastics was compared based on the behavioral indicators of Daphnia magna:

[0042] (1) The selected Daphnia magna had been cultivated continuously for one year under laboratory conditions. Specifically, Daphnia magna was cultured in reconstituted water configured with ultrapure water, and every 1000 mL of reconstituted water contained 58.5 mg of CaCl 2 2H 2 O, 24.7mg MgSO 4 ·7H 2 O, 13.0mg NaHCO 3 and 1.2mg KCl, and aerated for 24 hours before cultivation; cultivate Daphnia magna in a light incubator, set the temperature at 25±1°C, and the light-dark ratio 12h:12h. During the cultivation period, the reconstituted water was changed 3 times a week. Daphnia magna was fed with Scenedesmus obliques every day, and the feeding density was 10 5cells / mL; Healthy adult Daphnia magna individuals from the same female line, 3 generations of parthenogenetic reproduction, and 10 days old were selected for th...

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Abstract

The invention discloses a method for evaluating microplastic toxicity based on a daphnia magna behavioral index, which belongs to the technical field of emerging pollutant risk evaluation. The method comprises the following steps of (1) culturing, domesticating and selecting daphnia magna individuals, (2) preparing a micro-plastic exposure liquid, (3) respectively exposing daphnia magna in blank control and micro-plastic exposure liquid, (4) drawing a swimming behavior track of daphnia magna by using image processing software, and measuring behavioral indexes such as average speed (v) and acceleration (a), and (5) comparing and analyzing the behavior index change of daphnia magna under different exposure conditions so as to evaluate the toxicity level of the microplastics. According to the method, the toxicity of the micro-plastic can be preliminarily judged on the premise of less use of biochemical reagents and large analytical instruments, the workload is effectively reduced, and the method is simple and convenient to operate, high in practicability, low in cost and accurate and reliable in result.

Description

technical field [0001] The invention relates to a method for evaluating the toxicity of microplastics based on behavioral indicators of Daphnia magna, belonging to the technical field of emerging pollutant risk assessment. Background technique [0002] The annual output of plastic products worldwide has exceeded 300 million tons, and is growing at a rate of 20 million tons per year. Plastic waste has also become a global environmental problem. After long-term aging, the plastic waste entering the environment will further form microplastics with a size smaller than 5mm. The occurrence of microplastics in inland water bodies such as lakes, rivers, and reservoirs has been widely confirmed. It is called "PM2.5" in the water environment, and its water environment pollution has become a global environmental research hotspot. [0003] Microplastics in the aquatic environment may have toxic effects on aquatic organisms, causing potential ecological risks. At present, research on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/44
CPCG01N33/442
Inventor 丁剑楠刘舒娇邹华王震宇李祎飞张云付善飞
Owner JIANGNAN UNIV
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