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Method for determining toxicity of pesticide water dispersible granules by model biological alga

A technology of disperse particles and pesticide water, which is applied in the field of breeding, can solve the problems of lack of research data model suitability and reliability, and has not yet become a mature and recognized toxicity screening model. It is easy to reach the evaluation end point, the test period is short, and it is practical. strong effect

Active Publication Date: 2016-07-06
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few research reports on the toxicity screening of pesticide water-dispersible granules using algae. Even if there are some reports and related research models, there is a lack of relevant research data to evaluate the suitability and reliability of the model, and it has not yet become a mature and accepted model. Approved Toxicity Screening Models

Method used

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  • Method for determining toxicity of pesticide water dispersible granules by model biological alga
  • Method for determining toxicity of pesticide water dispersible granules by model biological alga
  • Method for determining toxicity of pesticide water dispersible granules by model biological alga

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Embodiment 1

[0027] A method for measuring the toxicity of pesticide fungicides by model organism algae of the present invention comprises the following steps:

[0028] S1. Preparation of test liquid:

[0029] BG11 medium dilution: Prepare the BG11 medium formula according to the formula in Table 1. The preparation method is: prepare the ingredients in Table 1 to make the corresponding concentration of mother liquor, and transfer the corresponding mother liquor dosage to a 1000mL volumetric flask in the order indicated , and distilled water to 1000mL, autoclaved at 121°C for 15 minutes, sealed and labeled, stored in a refrigerator at 4°C, valid for 2 months. Dilute the BG11 medium 10 times with distilled water to obtain the BG11 medium dilution.

[0030] Table 1: BG11 Medium Recipe

[0031]

[0032] Weigh 0.6667g of 75% carbendazim water-dispersible granule, dissolve it with BG11 medium dilution solution and settle it into a 100mL volumetric flask, and prepare the concentration to be ...

Embodiment 2

[0052] S1. Preparation of test liquid:

[0053] Weigh 0.1667g of 30% indoxacarb water dispersible granules, dissolve and settle to a 100mL volumetric flask with BG11 medium dilution (BG11 medium dilution is the same as in Example 1), to obtain a concentration of 500mga.i. / L For the stock solution, pipette 0.960, 2.400, 6.00, 15.00, 37.50mL of the stock solution in sequence, and dilute to 250mL volumetric flask with the BG11 culture medium dilution respectively, and prepare the concentration to be 1.92, 4.80, 12.0, 30.0, 75.0mga. i. / L of 2 times the drug solution.

[0054] S2, the preparation of algae dilution: measure the cell concentration of algae stoste (Sclerocystis cerevisiae) with hemocytometer method and be 4.00 * 10 6 , and diluted 100 times with sterile water, the cell concentration was 4.00×10 4 individual / mL.

[0055] S3, poisoning:

[0056] Experimental group: Take 50 mL of the experimental drug solution with gradient concentration prepared in step S1 and add i...

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Abstract

The invention discloses a method for determining the toxicity of pesticide water dispersible granules by model biological alga. The method comprises the following steps: preparing the pesticide water dispersible granules into a testing pesticide solution with a gradient concentration; diluting biological algae raw solution with sterile water to prepare an algae diluting solution; mixing the testing pesticide solution with the algae diluting solution; culturing at a sealed condition for 72h; counting up algae cells every 24h, and recording the biological effect of the algae cells and physicochemical parameters of the testing solution; calculating the median effective concentration EC50 on the algae cells of the pesticide water dispersible granules according to biological effect parameters of the algae cells; and judging the toxicity of the pesticide water dispersible granules. The method disclosed by the invention has the advantages of easiness in operation, strong practicability, short period and easiness in judging an evaluation finish point and the like.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a method for measuring the toxicity of pesticide water-dispersible granules by model algae. Background technique [0002] Algae are an important model organism for the study of important primary growers in ecosystems. As primary producers of aquatic ecosystems, algae occupy an important position. The diversity and quantity of its species will directly affect the structure and function of aquatic ecosystems. Many studies have used some sensitive algae species as indicator organisms, and these algae species have a great impact on It is sensitive to poison, easy to obtain, small in size and fast in reproduction, and can evaluate the impact of chemical substances on algae for many generations and population levels in a short period of time. It is a good experimental organism. It is widely used in the fields of life science, toxicology, environmental science and agricultural sci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 张德咏刘勇陈源蒋桂芳罗杰陈昂罗香文奚庆罗源华刘志邦严清平何安頔刘建宇
Owner HUNAN PLANT PROTECTION INST
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