A 3D Topography Restoration Method for Cell Structures Based on Area Constraints and Shape Constraints
A cell structure and area constraint technology, applied in the direction of graphic image conversion, image enhancement, image analysis, etc., can solve the problems of layer shape failure, difficult to reconstruct three-dimensional cell model, etc., to ensure authenticity, image authenticity and reliability, important Theoretical Significance and Effect of Practical Application Value
Active Publication Date: 2018-12-07
XI AN JIAOTONG UNIV
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In this method, the tomographic image of the cell collected by the microscope is used, but the shape of the layer close to the upper and lower sections of the cell is invalid, and it is difficult to reconstruct the three-dimensional model of the cell
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[0030] The present invention will be described in detail below in conjunction with the accompanying drawings. The present invention provides a cell three-dimensional morphology recovery method based on area constraints and shape constraints. In order to solve the problem of image blurring at the end, a method of 3D shape restoration is proposed, which completes the image restoration of the blurred layer, stitches the restored image and the confidence interval image in full space, and finally realizes the construction of a complete 3D space model of the cell structure.
[0031] The specific implementation modes provided by the present invention will be described in detail below in conjunction with the examples.
[0032] 1. Experimental sample preparation and fluorescence confocal acquisition
[0033] Cells: clinical human peripheral blood lymphocytes. The cell membrane was labeled with the fluorescent dye CM-Dil, and the nucleus was labeled with the fluorescent dye DAPI. Plac...
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The invention discloses a cellular structure (such as a cell nucleus and a cell membrane) three-dimensional morphology recovery method based on area constraint and shape constraint. Effective recovery of an organelle structure in a fluorescent confocal failure layer is achieved through the area constraint conditions and shape constraint conditions, and a cellular three-dimensional morphological structure model capable of being used for label-free cellular physical characteristics simulation calculation is finally obtained through splicing layer by layer. Compared with a common method that cellular incomplete three-dimensional morphology is directly reconstructed through an fluorescent confocal image, cellular morphology deletion generated by errors such as fluorescence labeling is effectively recovered, and the very important theoretical significance and actual application value on cellular intrinsic physical characteristic calculation and extraction which are achieved according to cellular morphology modeling and label-free inversion law research of cytopathic states are achieved.
Description
【Technical field】 [0001] The invention belongs to the research field of label-free cell detection structure modeling methods, and relates to a method for restoring the three-dimensional shape of cells in a tomographic failure layer based on area constraints and shape constraints. 【Background technique】 [0002] In the label-free cell detection method, the true internal and external morphology of suspension cells has a very close relationship with their corresponding intrinsic physical properties, such as scattering characteristics. Therefore, obtaining the complete true shape of the inside and outside of the cell is the basis and key to obtain its intrinsic physical characteristics. [0003] However, the commonly used fluorescent confocal tomography scanning method, due to incomplete fluorescent labeling of cell structures, imaging errors in tomographic scanning, and incomplete and missing tomographic images of cell structures, has led to the use of only fluorescent confocal...
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IPC IPC(8): G06F19/12G06T3/40G06T5/00G06T17/00
CPCG06T3/4038G06T5/001G06T17/00G06T2207/10072G06T2207/30024G16B5/00
Inventor 张璐李开兴解运浩赵欣
Owner XI AN JIAOTONG UNIV