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A technology for myoglobin and human myoglobin, which is applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of low measurement accuracy and complicated operation of myoglobin, and achieve good detection accuracy and detection sensitivity. High and convenient detection effect
Inactive Publication Date: 2016-08-31
ANHUI IPROCOM BIOTECH CO LTD
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[0005] The purpose of the present invention is to solve the problems of complex operation and low measurement accuracy in the myoglobin detection process in the prior art, and provide a kit for measuring myoglobin
Method used
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Embodiment 1
[0069] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein
[0070] Reagent R1:
[0071] Tris buffer 80 mmol / L
[0072] Sodium chloride 125 mmol / L
[0073] Polyethylene glycol-8000 13 g / L
[0074] Sodium azide 0.8 g / L
[0075] Bovine serum albumin 12~32 g / L
[0076] Its solvent is purified water.
[0077] Reagent R2:
[0078] Tris buffer 100 mmol / L
[0079] Bovine Serum Albumin 15 g / L
[0080] Glycerin 10 g / L
[0081] Sodium azide 0.8 g / L
[0082] Latex coated anti-human myoglobin antibody 3 g / L
[0083] Its solvent is purified water.
Embodiment 2
[0085] Kit preparation and method of use
[0086] 1. Preparation of latex-coated anti-human myoglobin antibody:
[0087] (a) Dilute latex particles with a particle size of 80nm to 3 g / L with 50 mmol / L MES buffer, add 1.0 mg of EDAC to each mL of solution, react at room temperature for 2 hours, and centrifuge at 15000 rpm Centrifuge at high speed for 30 minutes, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, and disperse by ultrasonic;
[0088] (b) Centrifuge the product of step (a) at 15,000 rpm for 30 minutes in a centrifuge, discard the supernatant, and suspend the precipitate in 50 mmol / L MES buffer, so that the final concentration of latex particles is 6.0 g / L, Ultrasonic dispersion, add an equal volume of MES diluent of anti-human myoglobin antibody while stirring, mix and stir, react at room temperature for 2 hours, the final concentration of latex particles is 3.0 g / L;
[0089] (c) Centrifuge the product of step (b) at 15,000 rpm for 30 minutes in...
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Abstract
The invention relates to the technical field of medicine and biochemistry and particularly relates to a kit for determining myoglobin. The kit is composed of double reagents R1 and R2, wherein the reagent R1 is prepared from the following components: 15mmol/L-145mmol/L of a Tris buffering solution, 50mmol/L-200mmol/L of sodium chloride, 5g/L-20g/L of polyethylene glycol-8000, 0.4g/L-1.2g/L of sodium azide, 12g/L-32g/L of bovine serum albumin and a solvent which is a purified water; the reagent R2 is prepared from the following components: 50mmol/L-150mmol/L of a Tris buffering solution, 4g/L-26g/L of bovine serum albumin, 5g/L-15g/L of glycerol, 0.6g/L-1.2g/L of sodium azide, 1g/L-4g/L of an emulsion-coated anti-human myoglobin antibody and a solvent which is purified water. Myoglobin (MYO) in the sample can be combined with the corresponding specific anti-MYO antibody in the reagent R2 to form an antigen-antibody complex and certain turbidity is generated. The turbidity level and the content of an antigen form a direct proportion when the certain antibody exists. The turbidity is determined under a certain wavelength and quantitative determination of the myoglobin can be carried out through a multi-point calibration curve. The kit has the advantages of high accuracy, convenient operation and the like.
Description
technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring myoglobin. Background technique [0002] Myoglobin (Mb) is an oxygen-binding heme protein, mainly distributed in cardiac muscle and skeletal muscle tissue, accounting for about 0.1% to 0.2% of the total muscle mass. In acute myocardial injury, Mb is first released into the blood. About 2-3 hours after the onset of symptoms, Mb in the blood can exceed the normal upper limit, reach the peak at 9-12 hours, and return to normal after 24-36 hours. Serial sampling is recommended for patients with suspected ACS because of the delay between the onset of symptoms and the release of protein markers into the blood. Negative Mb helps rule out myocardial infarction. [0003] Determination of serum myoglobin Myoglobin can be used as the most sensitive indicator for the early diagnosis of acute myocardial infarction (AMI). However, the specificity is po...
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