In Vitro Rapid Propagation Method of Virus-free Seedlings of Raspberry Palmifolia

Abstract

The invention relates to an in vitro rapid propagation method of raspberry palm-leaf virus-free seedlings, which belongs to the field of plant biotechnology, and comprises five steps of material extraction and sterilization, twig induction cultivation, root seedling induction cultivation, root seedling expansion cultivation and seedling hardening cultivation , using the method of root seedling growth, without the formation of callus, it can be subcultured from generation to generation in the propagation medium, which overcomes the asexual propagation methods such as cuttings, layering, ramets and root tillers in the prior art. The problems of low coefficient and relatively limited source of seedlings have also overcome the problems of yield decline and quality deterioration caused by the long-term use of asexual propagation in the prior art, thereby ensuring the excellent characteristics and characters of raspberry palmate and preventing varieties from being overgrown. It degenerates quickly, and has a short reproduction cycle, fast speed, and high coefficient. It is suitable for large-scale production of seedlings, and it is convenient to establish a production system for industrialized raspberry tissue culture seedlings. It provides a large number of high-quality seedlings for the raspberry palm industry in my country. Make up for the lack of palm raspberry market.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for in vitro rapid propagation of raspberry palm leaf virus-free seedlings. Background technique [0002] Palm-leaved raspberry (Rubus chingii Hu), commonly known as Jiaogong, Niuhai, etc., is a perennial berry fruit tree of the genus Rubus L. in the family Rosaceae. The fruit is picked when it is plump and green and immature. It can be used as a commonly used Chinese medicinal material. According to the records of the Compendium of Materia Medica, it has the effects of strengthening the kidney and stomach, replenishing essence, and treating kidney deficiency and enuresis. Its ripe fruit is a new type of high-nutrition fruit, rich in amino acids, sugars, organic acids, multivitamins, and mineral elements, especially the superoxide dismutase (SOD) content is the most in fruits, and it has the effects of anti-aging, health care, and beauty. "New third-generation fruit" ...

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned problems, the present invention provides a method for in vitro rapid propagation of raspberry palm leaf virus-free seedlings, so as to overcome the low reproduction coefficient caused by asexual propagation methods such as cuttings, layering, ramets and root tillers in the prior art. And the problem that the source of seedlings is greatly limited, also overcomes the problems of yield decline and quality deterioration caused by the long-term use of asexual propagation in the prior art, thereby ensuring the excellent characteristics and characters of palmate raspberry, preventing the species from degenerating too quickly, Moreover, the reproduction cycle is short, the speed is fast, and the coefficient is high. It is suitable for large-scale production of seedlings, and it is convenient to establish a production system for industrialized raspberry palmate tissue culture seedlings. Raspberry Market Shortcomings

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