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Pseudococcidae insect gene barcode detection kit and detection method thereof

A technology for detection kits and detection methods, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of insufficient system reports, meet the needs of high amplification efficiency, reduce sample quality, and simple operation Effect

Inactive Publication Date: 2016-09-07
SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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AI Technical Summary

Problems solved by technology

However, there are still not many systematic reports at home and abroad on the rapid detection technology of mealybug gene barcodes.

Method used

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  • Pseudococcidae insect gene barcode detection kit and detection method thereof
  • Pseudococcidae insect gene barcode detection kit and detection method thereof

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Embodiment Construction

[0035] The invention discloses a powdery scale insect gene barcode detection kit and a detection method thereof. The method can be an efficient, accurate and convenient molecular detection technology for powdery scale insects, and can distinguish 21 species of powdery scale insects at the molecular level , Can meet the needs of use.

[0036] A test kit for the gene barcode detection of mealybugs, which can quickly detect 21 species of mealybugs. The mealybugs are specifically shown in Table 1. The kit includes at least a gene barcode sequence disc and more than 20 primers PseJ / PseN reagent:

[0037] Primer PseJ sequence: 5'-CCTTCAACTAATCATAAAAATATNAG -3',

[0038] Primer PseN sequence: 5'-TAAACTTCT GGATGTCCAAAAAATCA-3';

[0039] The genetic barcode sequence inventory contains 93 standard barcode sequences, and the detailed sequences are shown in SEQ ID NO. 1 to SEQ ID NO. 93.

[0040] Table 1: Species and names of mealybugs

[0041] Serial number

Specimen name

Latin name

1

Pine...

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Abstract

The invention discloses a pseudococcidae insect gene barcode detection technology which is capable of quickly detecting 21 types of pseudococcidae. The pseudococcidae insect gene barcode detection technology at least comprises a primer PseJ / PseN reagent which can be used over 20 times and a gene barcode sequence disk; a primer PseJ sequence is 5'-CCTTCAACTAATCATAAAAATATNAG-3', and a primer PseN sequence is 5'-TAAACTTCT GGATGTCCAAAAAATCA-3'; 93 entries of standard barcode sequence are stored on the gene barcode sequence disk, and detailed sequences are as shown in SEQ ID NO.1 to SEQ ID NO.93. The pseudococcidae insect gene barcode detection technology is a highly effective, accurate and convenient pseudococcidae insect molecular detection technology, and is capable of distinguishing among 21 types of pseudococcidae insects at the molecular level, thereby satisfying the use demands.

Description

Technical field [0001] The invention relates to a PCR detection kit and a detection method thereof, in particular to a detection kit and a detection method for the gene barcode of a mealybug family insect, belonging to the technical field of plant quarantine. Background technique [0002] The mealybugs are a kind of herbivorous insects with a wide distribution and many hosts. Except for a few resource insects, most of them are common and serious pests in agriculture and forestry (Ben-Dov, 2002). Adults and nymphs of this group mainly feed on the young and tender parts of the host plant, leading to weak growth; it can also spread pathogenic microorganisms and cause plant diseases to occur in a large area; in addition, the honeydew discharged by the insects often induces the production of soot disease , It affects photosynthesis and has a huge ecological impact on agricultural and forestry production and urban landscape. [0003] For a long time, the operability and accuracy of the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/166
Inventor 郑斯竹杨晓军詹国辉
Owner SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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