Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability

Abstract

The invention discloses a castanopsis hystrix pollen collecting and storing method for increasing the germination rate and improving the pollen viability. The method comprises the following steps that when male parent male flowers are in a transitional period of an early blooming period and a full blooming period, male parent male flower inflorescence is collected at 9:00-11:00 or 15:00-17:00; the male flower inflorescence is dried for 12-24 h in a sterile room at the temperature of 22 DEG C-28 DEG C under illumination of 80 W-160 W, when a large amount of anther blows out and a large amount of yellow pollen scatters during gentle shaking, drying is completed; the dried male flower inflorescence is screened with a pollen sieve, pollen is collected and contained in a container, an opening of the container is sealed, and the container is placed in an environment at the temperature of minue 60 DEG C-minus 100 DEG C or liquid nitrogen to be stored. Furthermore, when the pollen needs to be germinated, the stored pollen is placed in a liquid culture medium and can be germinated at the temperature of 25 DEG C-35 DEG C. Accordingly, a castanopsis hystrix pollen collecting, drying, storing and viability detecting system is formed for the first time, the method is simple, rapid and high in operability, the castanopsis hystrix pollen viability can be improved, and the manual cross pollination success rate of castanopsis hystrix can be increased.

Description

technical field [0001] The invention belongs to the technical field of tree breeding. More specifically, it relates to a method for collecting and preserving red cone pollen that can increase germination rate and prolong pollen vigor. Background technique [0002] red cone ( Castanopsis hystrix A. DC) also known as Hongli, Chili, Limu, Castanopsis chinensis, Castanopsis chinensis, Castanopsis chinensis, Hongke, belongs to the evergreen tree of the Fagaceae Castanopsis genus, the tree body is straight, and the height of the tree can reach 30 meters. The diameter at breast height is more than 1 meter. It is the dominant species of evergreen broad-leaved forests in southern subtropical and subtropical vegetation in my country. Red cones are naturally distributed in east longitude 95o20′~118o0′, north latitude 18o30′~25o0′, the main production areas are concentrated in Guangdong, Guangxi, southern Fujian, and its surrounding areas are distributed in southern Yunnan, southeaster...

AI Technical Summary

Problems solved by technology

However, there are many kinds of forest trees and they are very different, which leads to complex and diverse forest tree hybrid breeding techniques. How to achieve the breeding goal of red cone with high quality is a big problem; compared with other tree species, the hybridization of red cone is more complicated. The reasons are: The inflorescence of the cone is relatively complex, consisting of pure male inflorescences, pure female inflorescences and bisexual inflorescences. The amount of pollen is large, the pollen grains are small and light, and they are very easy to be damp and agglomerated. The female stigma is a typical wet stigma. Natural pollination is prone to pollination failure. high rate of empty buds

Images