Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of probiotics and dunaliella salina tablets

The technology of probiotics and salina is applied in the field of preparation of probiotics salina tablets, which can solve the problems such as the lack of reports and documents, and achieve the effects of good antibiotic-related diarrhea, long survival period and good dosage form.

Inactive Publication Date: 2016-09-28
合肥赛为智慧医疗有限公司
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports and literatures on the preparation of biological products from probiotics and salina

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of probiotics and dunaliella salina tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of probiotic salina sheet comprises the following steps:

[0024] 1) Inoculate Clostridium tyrosilicate into the liquid fermentation medium at a volume ratio of 4%, anaerobically culture at 35° C. for 30 hours, and obtain a bacterium with a spore rate of more than 90%;

[0025] Then the bacterial liquid obtained from the fermentation culture was centrifuged at 2-6°C (rotational speed was 3000r / min, centrifugation time was 15min), the supernatant was removed, and the probiotic cells were collected;

[0026] The liquid fermentation medium is based on a liquid whey medium with a mass concentration of 8% as a base medium, then adding yeast extract powder to a mass concentration of 2%, adding peptone to a mass concentration of 2%; adding glucose to its mass concentration of 4%

[0027] 2) In parts by mass, 30 parts of salina powder, 4 parts of xylitol, 2 parts of mannitol, 8 parts of hydroxypropyl methylcellulose phthalate, 2 parts of polyethylene Py...

Embodiment 2

[0031] The preparation method of probiotic salina sheet comprises the following steps:

[0032] 1) Inoculate Bacillus coagulans into the fermentation medium at a volume ratio of 3%, and culture it anaerobically at a temperature of 37°C for 24 hours to obtain a bacterium with a spore rate of more than 90%;

[0033] Then the bacterial liquid obtained from the fermentation culture was centrifuged at 2-6°C (rotational speed was 3000r / min, centrifugation time was 15min), the supernatant was removed, and the probiotic cells were collected;

[0034] The liquid fermentation medium is based on a liquid whey medium with a mass concentration of 7% as a base medium, then adding yeast extract powder to a mass concentration of 2%, adding peptone to a mass concentration of 3%; adding glucose to its mass concentration of 2%

[0035] 2) In terms of parts by mass, 35 parts of salina powder, 4 parts of xylitol, 1 part of mannitol, 5 parts of hydroxypropyl methylcellulose phthalate, 1 part of po...

Embodiment 3

[0039] The preparation method of probiotic salina sheet comprises the following steps:

[0040] 1) Bifidobacteria are inoculated into the liquid fermentation medium at a volume ratio of 5%, anaerobically cultured at a temperature of 45°C for 36 hours, and then the bacterial liquid obtained from the fermentation culture is centrifuged at 2 to 6°C (rotating speed: 7000r / min, the centrifugation time is 20min), remove the supernatant, and collect the probiotic thallus;

[0041] The liquid culture medium is based on the liquid whey medium with a mass concentration of 20% as the base medium, then adding yeast extract powder to a mass concentration of 10%, adding peptone to a mass concentration of 5%; adding glucose to Its mass concentration is 8%;

[0042] 2) resuspending the collected probiotic cells with sterile purified water to obtain a probiotic suspension;

[0043] 3) In parts by mass, 40 parts of salina powder, 5 parts of xylitol, 5 parts of mannitol, 20 parts of hydroxypro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of probiotics and dunaliella salina tablets. The method comprises the following steps that 1, probiotics for health-care foods are inoculated into a liquid fermentation medium, anaerobic culture, centrifugal separation and supernatant removing are conducted, and probiotics thalli are collected; 2, the collected probiotics thalli are subjected to resuspension with sterile purified water, and a probiotics suspension is obtained; 3, dunaliella salina powder, xylitol, mannitol, hydroxypropylmethyl cellulose phthalate, polyvinylpyrrolidone and aspartame are fully mixed, smashed and sieved, and dunaliella salina tablet coarse powder is obtained; 4, the dunaliella salina tablet coarse powder is added to the probiotics suspension, the dunaliella salina tablet coarse powder and the probiotics suspension are mixed, granulated and dried, and intermediate granules are obtained; 5, magnesium stearate, silicon dioxide and the intermediate granules are mixed to be uniform, tabletting is conducted, and the probiotics and dunaliella salina tablets are obtained.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a preparation method of probiotic algae salina tablets. Background technique [0002] Salina is also known as Dunaliella, and the genus Dunaliella belongs to the Chlorophyta (Chlorophyta), Vovocales (Vovocales) Dunaliellaceae (Dunaliellaceae). Dunaliella is a unicellular planktonic microalgae with no cell wall composed of cellulose outside the cell and only covered by a thin and elastic membrane. Dunaliella salina has a very wide range of salt tolerance (NaCl concentration higher than 30%) and a wide range of pH tolerance, and can grow in the range of pH5-11, and the optimum NaCl concentration is 22%; light-seeking, strong acid resistance Alkali, high cold (-27°C) and extreme heat (+53°C). [0003] Dunaliella has a lower ash content than other green algae and a higher protein and lipid content. Salina polysaccharide is a general term for a series of polysaccharides...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/05A61K9/20A61P1/14A61P1/12A61P1/04A61P1/16A61P35/00A61P1/00A23L33/135A23L33/105A61K35/744A61K35/747A61K35/745A61K35/742A61K35/741
CPCA61K36/05A23V2002/00A61K9/2054A61K35/741A61K35/742A61K35/744A61K35/745A61K35/747A61K2300/00A23V2200/3204A23V2200/32A23V2200/308
Inventor 郭亮王盛洲何学松彭继博肖彩云韩菲钱圆
Owner 合肥赛为智慧医疗有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com