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Method for producing biodiesel from chlorella

A biodiesel and chlorella technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of increasing production, stabilizing the accumulation of oil, improving and improving the quality of oil

Inactive Publication Date: 2016-10-05
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on microalgae biodiesel at home and abroad is mainly focused on how to increase the oil content and how to catalyze the conversion of oil into biodiesel. In the cultivation stage of microalgae, how to improve the oil content while improving The quality of oil has almost become blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment a

[0078] The present embodiment provides a kind of method that produces biodiesel with chlorella, and its step comprises:

[0079] 1) Inoculate the sterile chlorella seeds in a closed photobioreactor to expand and re-cultivate. The specific steps are: inoculate the triangular shaker flask containing vulgaris chlorella into the sterile photobioreactor. Light culture in the culture medium of good bacteria; the photobioreactor is a bubbling closed reactor, including CO 2 Gas storage cylinder, gas mixing device and gas flow meter; the light source is LED light, the light intensity is 8Klux, and the light quality is white light; the composition of the seed medium is: sea salt 24g / L, CH 3 COONa 0.3g / L, NaNO 3 200mg / L, NaH 2 PO 4 50mg / L, Na 2 EDTA 10mg / L, FeSO 4 ·7H 2 O 2.5mg / L, vitamin B1 0.006mg / L, vitamin B2 0.00005mg / L; initial pH of culture was 6.0, ventilation intensity was 0.2VVM, CO 2 The concentration is 0%, the culture temperature is 23°C, culture for about 3 days, unt...

Embodiment b

[0096] The present embodiment provides a kind of method that produces biodiesel with chlorella, and its step comprises:

[0097] 1) Inoculate the sterile chlorella seeds in a closed photobioreactor to expand and re-cultivate. The specific steps are: inoculate the triangular shaker flask containing vulgaris chlorella into the sterile photobioreactor. Light culture in the culture medium of good bacteria; the photobioreactor is a bubbling closed reactor, including CO 2 Gas storage cylinder, gas mixing device and gas flow meter; the light source is a fluorescent lamp, the light intensity is 10Klux, and the light quality is white light; the composition of the seed medium is: sea salt 34g / L, CH 3 COONa 0.5g / L, NaNO 3 300mg / L, NaH 2 PO 4 100mg / L, Na 2 EDTA 10mg / L, FeSO 4 ·7H 2 O 5.0mg / L, vitamin B1 0.06mg / L, vitamin B2 0.0005mg / L; initial pH of culture was 7.5, ventilation intensity was 0.7VVM, CO 2 The concentration is 0.5%, the culture temperature is 26°C, culture for about ...

Embodiment c

[0105] The present embodiment provides a kind of method that produces biodiesel with chlorella, and its step comprises:

[0106] 1) Inoculate the sterile chlorella seeds in a closed photobioreactor to expand and re-cultivate. The specific steps are: inoculate the triangular shaker flask containing vulgaris chlorella into the sterile photobioreactor. Light culture in the culture medium of good bacteria; the photobioreactor is a bubbling closed reactor, including CO 2 Gas storage cylinder, gas mixing device and gas flow meter; the light source is LED light, the light intensity is 9Klux, and the light quality is white light; the composition of the seed medium is: sea salt 28g / L, CH 3 COONa 0.4g / L, NaNO 3 240mg / L, NaH 2 PO 4 60mg / L, Na 2 EDTA 10mg / L, FeSO 4 ·7H 2 O 3.0mg / L, vitamin B1 0.01mg / L, vitamin B2 0.0001mg / L; initial pH of culture was 6.5, ventilation intensity was 0.4VVM, CO 2 The concentration is 0.2%, the culture temperature is 24°C, culture for 4 days, until the...

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Abstract

The invention discloses a method for producing biodiesel from chlorella. The method comprises the following steps: (1) seed culture; (2) seed collection; (3) photoautotrophic cultivation; (4) harvesting of microalgae; and (5) oil extraction. The method provided by the invention uses common chlorella and conditions like mild illumination and light quality, sufficient nutrients, a proper nitrogen-phosphorus ratio and proper temperature and can promote rapid growth of chlorella cells, accumulation of oil and stabilization and improvement of oil quality.

Description

technical field [0001] The invention relates to microalgae energy technology, in particular to a method for producing high-quality biodiesel from chlorella. Background technique [0002] As a substitute for petrochemical diesel, microalgae biodiesel should meet the requirements for the use of petrochemical diesel, that is, its physical and chemical properties should be close to those of petrochemical diesel to ensure its fuel characteristics. The indicators to measure the properties of biodiesel mainly include: density, kinematic viscosity, flash point, slip distance, low temperature performance, carbon residue, ash, cetane number, methanol content, lipid content, phosphorus content, glycerin content, glycerin content, iodine value And free fatty acids, etc., wherein the combustion performance of biodiesel is evaluated by cetane number. Combustion performance is one of the most important qualities of biodiesel, while cetane number is mainly affected by raw materials. In ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/10
Inventor 孙洪磊李建忠齐泮仑何皓郑蕾雪晶张家仁孙立明李萌萌张佳
Owner PETROCHINA CO LTD
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