Buffer solution for increasing success rate of preparing chromosome slide

Abstract

The invention discloses a buffer solution for increasing the success rate of preparing a chromosome slide. The buffer solution is prepared from the following components in parts by weight: 3 to 4 parts of potassium dihydrogen phosphate, 14 to 16 parts of disodium hydrogen phosphate and 2000 parts of water by supplementing the surplus. A preparation method of the buffer solution comprises the following steps: S1, keeping constant temperature of a preparation room, weighing the potassium dihydrogen phosphate and the disodium hydrogen phosphate in corresponding parts by weight, respectively adding water for dissolving, transferring the dissolved potassium dihydrogen phosphate and the dissolved disodium hydrogen phosphate in the same volumetric flask, and diluting by using water, thus obtaining a mixed solution; S2, regulating the pH (Potential of Hydrogen) value to be 7 to 8.5 by using phosphoric acid; S3, carrying out high-pressure sterilization, and transferring the mixed solution into a reagent bottle after cooling to room temperature, thus obtaining the buffer solution for increasing the success rate of preparing the chromosome slide, wherein the buffer solution is sealed and preserved in a refrigerator of which the temperature is 0 to 4 DEG C for standby use. The buffer solution provided by the invention is capable of effectively increasing the success rate of preparing a peripheral chromosome slide, increasing the working efficiency, reducing the re-dripping rate of the chromosome slide, reducing the workload of experimenters, reducing the loss of a reagent and reducing the slide preparation cost.

Description

Technical field [0001] The present invention relates to the technical field of buffer solutions, in particular to a buffer solution for improving the success rate of preparing chromosome slides. Background technique [0002] In biology and botany experiments, it is often necessary to make chromosomes. In the process of making peripheral blood chromosomes, it is often encountered that cells and chromosomes have a blue background, which affects the effect of filming and the success rate of filming. In the process of chromosome preparation, the re-dropping rate of chromosome slides is generally high, resulting in a lower success rate of chromosome preparation, which not only causes the loss of experimental reagents, but also increases the workload of the experimenters, and also delays the progress of the experiment and reduces work effectiveness. Summary of the invention [0003] The purpose of the present invention is to solve the shortcomings in the prior art, and proposes a buffe...

AI Technical Summary

Problems solved by technology

[0002] Chromosome preparation is often required in biology and botany experiments. In the process of peripheral blood chromosome preparation, cells and chromosomes often have a blue background, which affects the effect of preparation and the success rate of preparation. During the process of chromosome preparation, the heavy drop rate of chromosome slides is generally high, resulting in a low success rate of chromosome preparation, which not only causes the loss of experimental reagents, but also increases the workload of experimenters, and at the same time delays the progress of the experiment and reduces the workload. efficiency