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A kind of plant polyphenol extract of the genus Columbine and preparation method thereof

A technology of plant polyphenols and extracts, applied in plant raw materials, plant/algae/fungus/moss components, pharmaceutical formulations, etc.

Inactive Publication Date: 2020-06-12
SICHUAN NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far there has been no match for Related reports on the maximum extraction and further separation and purification of polyphenols in genus plants

Method used

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  • A kind of plant polyphenol extract of the genus Columbine and preparation method thereof
  • A kind of plant polyphenol extract of the genus Columbine and preparation method thereof
  • A kind of plant polyphenol extract of the genus Columbine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The present invention Preparation of genus plant polyphenol extract

[0044] Accurately weigh the grinded stick Docynia indica (Wall.) Dcne. leaf 1kg, ultrasonic power 800W, ultrasonic frequency 40KHz, with 63% ethanol at a liquid-to-material ratio of 20:1 (mL / g), extract at 65 ° C for 41 min, extract twice, and combine the extracts , concentrated to obtain crude extract;

[0045] Pretreatment of D-101 macroporous adsorption resin: After sealing and soaking the macroporous resin D-101 in a 95% ethanol aqueous solution for 24 hours, rinse it with ultrapure water until there is no alcohol smell; then use 5% HCl solution Seal and soak for 2 to 4 hours, rinse with ultrapure water until neutral; finally, seal and soak with 2% NaOH solution for 2 to 4 hours, rinse with ultrapure water until neutral, and set aside for later use;

[0046] The crude extract was taken and loaded on the pretreated D-101 macroporous adsorption resin. The mass concentration of the ...

Embodiment 2

[0047] Embodiment 2 The present invention Preparation of genus plant polyphenol extract

[0048] Accurately weigh the grinded stick Docynia indica (Wall.) Dcne. leaf 1kg, ultrasonic power 800W, ultrasonic frequency 40KHz, with 63% ethanol at a liquid-to-material ratio of 20:1 (mL / g), extract at 65 ° C for 41 min, extract twice, and combine the extracts , concentrated to obtain crude extract;

[0049] Pretreatment of D-101 macroporous adsorption resin: After sealing and soaking the macroporous resin D-101 in a 95% ethanol aqueous solution for 24 hours, rinse it with ultrapure water until there is no alcohol smell; then use 5% HCl solution Seal and soak for 2 to 4 hours, rinse with ultrapure water until neutral; finally, seal and soak with 2% NaOH solution for 2 to 4 hours, rinse with ultrapure water until neutral, and set aside for later use;

[0050] The crude extract was taken and loaded on the pretreated D-101 macroporous adsorption resin. The mass concentration of the ...

experiment example 1

[0052] Experimental Example 1 Comparison of factors of ultrasonic extraction process Effect of polyphenol extraction rate

[0053] 1. Materials, reagents and instruments

[0054] Materials and Reagents

[0055] 栘 The leaves of Docynia indica (Wall.) Dcne. were collected in Xichang City, Sichuan Province in April 2014. They were identified by Professor Luo Qiang of Xichang College and were in line with the characteristics of this species.

[0056] Phloridzin reference substance (HPLC>98%, purchased from Chengdu Plant Standard Chemical Co., Ltd., Sichuan Province); Folin-Ciocaileus reagent (FC reagent, purchased from Shanghai Ruji Biotechnology Co., Ltd.); ethanol (analytical grade), Anhydrous sodium carbonate (analytical grade) (purchased from Chengdu Kelong Co., Ltd.).

[0057] instrument

[0058] equipment name Model or Specification Manufacturer UV spectrophotometer UV-1700 Shimadzu, Japan Vacuum freeze drying oven wizard 2.0 American V...

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Abstract

The invention discloses a Docynia delavayi polyphenol extract which has polyphenol content of 91.24 to 91.34% (w / w). The invention also provides a preparation method for the extract. With extraction conditions provided in the invention, polyphenol substances can be maximally extracted from Docynia delavayi, and an extraction rate is as high as 18.54%. High-purity polyphenol and phlorizin can be obtained by further purifying a crude extract with the purifying method provided by the invention. Pharmacological experiments show that the Docynia delavayi polyphenol extract can substantially reduce the levels of cholesterol TC, triglyceride TG, high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in the serum of mice; thus, the Docynia delavayi polyphenol extract can effectively prevent and treat diseases related to abnormal lipids metabolism, such as obesity resulting from abnormal blood fat content, hyperlipidemia, atherosclerosis and coronary heart diseases.

Description

technical field [0001] The present invention relates to a The invention belongs to a plant polyphenol extract and a preparation method thereof, and belongs to the fields of functional food and medicine. Background technique [0002] 栘 Rosaceae The general name of the genus, there are three species: Yunnan Cone Docynia delavayi (Franch.) Schneid, 栘 Docynia indica (Wall.) Dcne. and Longclaw Docynia longiunguisQ.Luo et J.L.Liu, mainly distributed in Yunnan, Guichuan and other places, among which Yunnan It is a famous local wild fruit. [0003] Currently, for the The research and development of the genus is still in its infancy. The basic research on the genus is still very insufficient, and the research on its active ingredients is very little, resulting in a large waste of resources. Liu Haixia et al. improved the traditional Folin-Ciocalteu colorimetric method and established a highly exclusive colorimetric method. Method for the determination of polypheno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P3/06A61P3/04A61P9/10
CPCA61K36/73A61K2236/333A61K2236/39A61K2236/55
Inventor 张晓喻刘刚王战国严春新任兰杨军
Owner SICHUAN NORMAL UNIV
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