Bionic magnetosome loaded with siRNA and preparation method of magnetosome

A magnetosome and bionic technology, applied in the fields of chemistry and biomedicine, can solve problems such as cytotoxicity

Active Publication Date: 2016-10-26
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The long cycle inside the biological organism can be realized by modifying or modifying the surface of the carrier system. At present, most of the surface modification, such as polyethylene glyc

Method used

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  • Bionic magnetosome loaded with siRNA and preparation method of magnetosome
  • Bionic magnetosome loaded with siRNA and preparation method of magnetosome
  • Bionic magnetosome loaded with siRNA and preparation method of magnetosome

Examples

Experimental program
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Example Embodiment

[0122] Example 1

[0123] A method for preparing biomimetic magnetosomes loaded with siRNA, the specific steps of the method are as follows:

[0124] Step 1: Preparation of MNC

[0125] (1) Put DEG in an Ar atmosphere. After the oxygen is completely removed, dissolve solid NaOH into DEG, heat at the same time, and increase the temperature to 120°C. Stop heating until the solid NaOH is completely dissolved. The preparation process is all used Ar gas protection, prepare a light yellow solution, which is a NaOH storage solution, keep it at 70°C for later use;

[0126] (2) The FeSO 4 ·7H 2 O, PEI and the solvent are evenly mixed and vacuumed for 25 minutes, and then filled with Ar gas protection; heating to 160 ℃, adding the NaOH storage solution obtained in step 1 (1), until the molar concentration of NaOH in the solution is 0.03mol / L, about After 3 minutes, the color of the solution changed to black, then the temperature was raised to 220°C, and the reflux was continued for 1 hour. Aft...

Example Embodiment

[0188] Example 2

[0189] A method for preparing biomimetic magnetosomes loaded with siRNA, the specific steps of the method are as follows:

[0190] Step 1: Preparation of MNC

[0191] (1) Put DEG in an Ar atmosphere, after the oxygen is completely removed, dissolve solid NaOH into DEG, heat at the same time, and raise the temperature to 100°C until the solid NaOH is completely dissolved and stop heating. The preparation process is used throughout Ar gas protection, prepare a light yellow solution, which is a NaOH storage solution, keep it at 70°C for later use;

[0192] (2) Add FeCl 2 ·4H 2 O, PEI and the solvent are evenly mixed and vacuumed for 25 minutes, and then filled with Ar gas protection; heating to 100 ℃, adding the NaOH storage solution obtained in step 1 (1) until the molar concentration of NaOH in the solution is 0.03 mol / L, about After 3 minutes, the color of the solution turned black, then the temperature was raised to 190°C, and the reflux was continued for 30 minut...

Example Embodiment

[0208] Example 3

[0209] A method for preparing biomimetic magnetosomes loaded with siRNA, the specific steps of the method are as follows:

[0210] Step 1: Preparation of MNC

[0211] (1) Put DEG in an Ar atmosphere. After the oxygen is completely removed, dissolve solid NaOH into DEG, heat at the same time, and increase the temperature to 150°C. Stop heating until the solid NaOH is completely dissolved. The preparation process is all used Ar gas protection, prepare a light yellow solution, which is a NaOH storage solution, keep it at 70°C for later use;

[0212] (2) The FeSO 4 ·7H 2 O, PEI and the solvent are evenly mixed and vacuumed for 25 minutes, and then filled with Ar gas protection; heated to 180 ℃, add the NaOH storage solution obtained in step 1 (1), until the molar concentration of NaOH in the solution is 0.4 mol / L, about After 3 minutes, the color of the solution changed to black, then the temperature was raised to 230°C, and the reflux was continued for 2 hours. The re...

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Abstract

The invention relates to a bionic magnetosome loaded with siRNA and a preparation method of the magnetosome, and belongs to the field of chemistry and biomedicine. According to the bionic magnetosome, water-soluble MNC serves as the kernel and is wrapped by a white cell membrane, and siRNA is loaded between the MNC and the white cell membrane; antibodies or peptides with modifying groups can be coupled outside the white cell membrane of the bionic magnetosome in a covalence mode. By preparing MNC, M and MNC:siRNA and mixing an MNC:siRNA solution, an M solution and a buffer solution, the bionic magnetosome is obtained; the white cell membrane of the bionic magnetosome is modified by the peptides or antibodies through the modifying groups, and the bionic magnetosome coupled with the antibodies or peptides is obtained. The MNC in the bionic magnetosome has positive charges, can adsorb siRNA and is wrapped by M for bionics, a focus is reached efficiently and rapidly, and meanwhile immune elimination of organisms is avoided.

Description

technical field [0001] The invention relates to a biomimetic magnetosome loaded with siRNA and a preparation method thereof, belonging to the fields of chemistry and biomedicine. Background technique [0002] RNA interference (RNAi) refers to the specific gene silencing after inducing transcription of exogenous or endogenous double-stranded small RNA (siRNA) homologous to the target gene in organism cells. RNAi drugs have shown great application prospects in tumors, genetic diseases and other diseases, but so far, RNAi technology still has siRNA targeting and off-target effects, in vivo instability, interferon effects, and drug delivery in the treatment of tumors. System, delivery method and drug safety and other issues. [0003] Nanocarrier-mediated gene transfection has advantages that other drug carriers do not have, especially magnetic nanoparticles have good biocompatibility, superior magnetic properties and high stability, and can be used for magnetically targeted siR...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/713A61K47/48A61K47/46A61K47/34A61K41/00A61P35/00
Inventor 谢海燕张帆马光辉魏炜赵龙剑
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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