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Device integrating sampling, reaction and detection

A reaction and integrated technology, applied in the direction of measuring device, sampling device, test sample preparation, etc., can solve the problems of not being able to use a variety of reagents integrated device, liquid can not enter, etc., to achieve simple structure, convenient operation, avoid sample pollution effect

Active Publication Date: 2016-10-26
益思美诠生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the valve needle is broken, the liquid cannot enter the inner cavity of the sampling swab
Due to the structure of the device, only one reagent can be contained in the liquid storage tube, and it cannot be applied to an integrated device for multiple reagent reactions, and its use has certain limitations.

Method used

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  • Device integrating sampling, reaction and detection
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 of the present invention: color reaction of enzyme and substrate

[0028] like figure 1 As shown in h, the device in Example 1 can be applied to the color reaction between enzyme and substrate. Before the reaction, the enzyme and the substrate should be stored separately and mixed during the reaction. Meanwhile, in some cases, the substrates of certain enzymes are unstable at normal temperature in liquid state, but their solid state (powder form) can be stored stably at normal temperature. At this time, the buffer solution containing enzyme is placed in the storage cavity formed by the liquid separation valve 4 and the liquid storage tube 1, and the corresponding substrate (powdered form) of the enzyme is placed in the gap A between the liquid separation valve 4 and the sampling swab rod fixing piece 7 or solid state), and separated by porous filter membrane 6 (porous filter membrane 6 is located in the gap A, close to the sampling swab rod 8 fixed piece ...

Embodiment 2

[0029] Embodiment 2 of the present invention: culture of fastidious microorganisms

[0030] like figure 1 As shown in i, the device in Example 2 can be applied to the cultivation of fastidious microorganisms. Fastidious organisms, such as Neisseria, were found in clinically tested specimens. This type of microorganism has stricter requirements on the environment for transportation and cultivation, and requires the cultivation environment to contain 5%-10% carbon dioxide. At the same time, after the sample detection is completed, in order to avoid the sample from polluting the environment, a bactericide can be added to the device. like figure 1 As shown in i, the bactericide is placed in the ampoule tube 2, the citric acid solution is placed in the liquid storage tube 1, separated by the liquid separation valve 4, and the carbonic acid solution is placed in the gap A between the liquid separation valve 4 and the sampling swab rod fixing piece 7. Sodium powder, separated by ...

Embodiment 3

[0031] Embodiment 3 of the present invention: sample pretreatment

[0032] like figure 2 As shown in a, the device in Example 3 can be applied to the pretreatment of similar whole blood samples. like figure 2 As shown in a, the liquid colloidal gold can be placed in the storage cavity formed by the liquid separation valve 4 and the liquid storage tube 1, and a cell lysis reagent (such as TritonX -100), separated by porous filter membrane 6 (porous filter membrane 6 is located in the gap A, close to the sampling swab rod fixed piece 7, see figure 2 a). When in use, rotate out the joint between the liquid storage tube 1 and the reaction tube 10, and take out the sampling swab rod 8 for sampling. After the sample is collected, the sampling swab rod 8 is inserted back into the reaction tube 10 and tightly screwed with the liquid storage tube 1 . Secondly, break off the valve needle 5 of the liquid separation valve 4, release the liquid colloidal gold to flow through the ga...

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Abstract

The invention discloses two devices integrating sampling, reaction and detection. A first device comprises a liquid storage tube, a liquid distribution valve, a fixing sheet, a sampling swab rod and a reaction tube, wherein a breakable valve needle is arranged on the liquid allocation valve; the fixing sheet is connected with the sampling swab rod; the liquid allocation valve is arranged inside the inner cavity of the liquid storage tube; the storage cavity formed by the liquid storage tube and the liquid distribution valve is filled with a solution; the valve needle of the liquid allocation valve is positioned inside the storage cavity; the fixing sheet is arranged inside the liquid storage tube; a gap is formed between the fixing sheet and the liquid distribution valve; the gap is filled with chemical substances; the opening end of the liquid storage tube is sleeved by the reaction tube; a second device contains all elements of the first device, and further contains a damageable ampoule tube; the valve needle of the liquid allocation valve is positioned outside the storage cavity. The devices are sample in structure, multiple reagents can be placed in different zones and are mixed to use before reaction, and steps of sampling, treatment, reaction and detection on samples can be continuously completed. The devices can be widely applied to pathogen detection, microculture, enzyme activity analysis, immunochemistry detection and the like of clinical samples.

Description

technical field [0001] The invention relates to a chemical device, in particular to a device integrating sampling, reaction and detection. Background technique [0002] The field of medical testing involves two parts, sample collection and testing. Due to the lack of an effective reaction device integrating sampling and detection, the above two steps are currently carried out separately. At the same time, the reliability of the test results depends on the timely and effective processing of samples, so the integrated device integrating sampling, reaction and detection can process samples at the sampling site, which not only saves time but also improves the detection accuracy accordingly . [0003] The Chinese invention patent with the patent number ZL200510023792.5 discloses a device for sample collection and testing. The device contains a breakable valve, and the valve needle of the breakable valve is located in the liquid storage bag solution. At the same time, the valve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/02G01N1/28G01N21/78G01N33/00
CPCG01N1/02G01N1/28G01N21/78G01N33/00
Inventor 陈国治覃冰
Owner 益思美诠生物科技(上海)有限公司
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