High-flux micro-fluidic chip nucleic acid amplification analysis detection system

Abstract

The invention relates to a high-flux micro-fluidic chip nucleic acid amplification analysis detection system which is characterized by comprising a revolution-rotation composite movement platform, micro-fluidic chips, a multi-axis movement control system, a multi-way PID temperature control system, an optical detection system and a data acquisition processing and display system, wherein more than two revolution movement positioning shafts are arranged at circumference graduation intervals on the revolution-rotation composite movement platform; each micro-fluidic chip is fixedly arranged on the revolution movement positioning shaft of the revolution-rotation composite movement platform; the multi-axis movement control system is used for controlling movement of the revolution-rotation composite movement platform; the multi-way PID temperature control system is used for adjusting and controlling the temperature of each micro-fluidic chip; the optical detection system is used for detecting the high sensitivity of a trace sample placed inside each micro-fluidic chip in real time, and used for transmitting the detection result to the data acquisition processing and display system in real time. By adopting the high-flux micro-fluidic chip nucleic acid amplification analysis detection system, the high-flux nucleic acid amplification detection application requirement of dozens to two hundred of samples every day can be met.

Description

technical field [0001] The invention relates to a nucleic acid detection system, in particular to a high-throughput microfluidic chip nucleic acid amplification analysis and detection system. Background technique [0002] Nucleic acid amplification analysis is an advanced biomedical analysis method widely used in the twentieth century, which can copy specific sequence nucleic acid fragments to 10 in about 2 hours 6 ~10 9 copy number, enabling highly sensitive molecular identification. The existing nucleic acid amplification analysis mainly includes two types: variable temperature amplification and isothermal amplification. The variable temperature amplification method (Polymerase chain reaction, PCR) is the earliest nucleic acid amplification technology. The nucleic acid amplification reaction is started by completely matching the positive and negative two specific primers, and the cycle is carried out in units of amplification cycles. Each amplification cycle is about 90 ...

AI Technical Summary

Problems solved by technology

However, the above-mentioned system can only detect one micro-nanoliter system fluid chip at a time. According to the calculation of 8 hours of work a day, one instrument can only detect less than 8 micro-nanoliter system fluid chips a day, even if it works overtime in the morning and evening. In the case of hours, at most 10 samples can be tested a day, which cannot meet the needs of nucleic acid analysis applications with dozens or even hundreds of samples per day in medium-sized hospitals

Images