Enterobacter cloacae and its use

A technology of Enterobacter cloacae, which is used in the field of inhibiting the growth of red tide algae, can solve the problems of coastal economy and aquaculture loss, etc., and achieve the effect of improving the treatment effect and improving the effect

Active Publication Date: 2019-07-09
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some red tide organisms are also accompanied by toxins, which directly poison aquatic organisms and bring huge losses to the coastal economy and aquaculture industry

Method used

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  • Enterobacter cloacae and its use
  • Enterobacter cloacae and its use
  • Enterobacter cloacae and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Screening, identification and characteristics of embodiment 1 bacterial strain

[0050] 1. Screening of strains

[0051] On August 21, 2015, a localized red tide occurred near the coast of the South China Sea (Shenzhen Yantian Port, surface water area). Karenia mikimotoi and Scrippsiella trochoidea. Retrieve the water sample from this area, bring the water sample back to the laboratory and filter it through gauze to remove impurities and large particles in the water; then filter it through a 100μm metal mesh to remove debris in the water; finally filter it through a 10μm filter membrane to remove algae cells , and the filtrate was coated and screened for bacteria.

[0052] The filtrate prepared above was diluted in multiple ratios, and 20 μL were respectively spread on the 2216E seawater solid medium (recipe: peptone 5.0g, yeast extract 1.0g, iron phosphate 0.01g, aged seawater 1000mL, agar 20g, adjust the pH to 7.8), cultivate overnight until clear single colonies g...

Embodiment 2

[0065] The detection of embodiment 2 strain producing QS ability

[0066] 1. Preparation of Enterobacter cloacae strains. The identified Enterobacter cloacae.ST3 was cultured in 1.5 milliliters of eppendof tubes for 6 to 8 hours, the amount of liquid in the eppendof tubes was 0.5 milliliters, and the culture medium was LB liquid medium (the formula of the culture medium was: tryptone 10g, Yeast extract 5g, NaCl 10g, adjust pH to 7.0. Dilute to 1L with deionized water. Steam sterilization under 15psi high pressure for 20min). The concentration of the cultured bacterial solution is detected by a visible light spectrophotometer, and the concentration value is about OD 600 = 0.3. The prepared bacterial solution was stored in a 4°C refrigerator for later use.

[0067] 2. Preparation and detection of test strains. QS produced by Enterobacter cloacae.ST3 was detected with a reporter strain (Agrobacterium tumefaciens, A136) that specifically detects QS. The detection method is as...

Embodiment 3

[0070] Example 3 Application of Enterobacter cloacae.ST3 in inhibiting algae

[0071] 1. Method of inhibiting algae by Enterobacter cloacae.ST3

[0072] 1. High-density culture of Enterobacter cloacae.ST3

[0073] The Enterobacter cloacae bacterial strain Enterobacter cloacae.ST3 that is obtained by embodiment 1 is carried out primary culture (2216E liquid medium, formula: peptone 5.0g, yeast extract 1.0g, ferric phosphate 0.01g, aged seawater 1000mL, adjusted in 50mL Erlenmeyer flask pH to 7.8), the culture condition is 30°C, the rotation speed is 200r / min, and the time is 6h, when the strain density is close to OD 600 =0.5, transfer to a 250mL Erlenmeyer flask for secondary culture. Continue to cultivate for 18 hours until the strain density reaches 1×10 9 Individual / mL concentration, spare.

[0074] 2. Cultivation of algae cells for experiments

[0075] The algal species used in the experiment was Gyrodinium instriatum. Take the algae subcultured in the laboratory (th...

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Abstract

The present invention proposes an Enterobacter cloacae and its use, and was deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee on March 11, 2016, with the preservation number CGMCC12206. The Enterobacter cloacae was isolated and discovered by the inventor in the ocean for the first time, and has a remarkable effect in controlling red tides, treating water samples containing red tide algae and inhibiting the growth of red tide algae.

Description

technical field [0001] The invention relates to the field of biology, in particular, the invention relates to Enterobacter cloacae, the use of Enterobacter cloacae, a method for controlling red tide, a water treatment method and a method for inhibiting the growth of red tide algae. Background technique [0002] Red tide refers to the ecological phenomenon in which plankton (especially algae) explodes in a short period of time or highly gathers in the ocean, causing abnormal water color (mostly red or brown), which is called "algal bloom" or "red tide". ghost". The increasing pollution of the offshore environment and the unreasonable development of marine resources have brought huge pressure on the ecological environment, resulting in frequent occurrence of local red tides and ecological disasters in the marine environment. This ecological disaster has caused water pollution, energy consumption, and the reduction of dissolved oxygen in water, posing a serious threat to the l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12P1/04C12R1/01
CPCC02F3/34C12P1/04C12N1/205C12R2001/01
Inventor 周进
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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