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A plasma miRNA marker related to the auxiliary diagnosis of IGA nephropathy and its application

An auxiliary diagnosis and plasma technology, applied in the field of genetic engineering nephrology, can solve the problems of incomplete research results and no miRNA detection method, and achieve the effect of high sensitivity and specificity, easy access and good stability

Active Publication Date: 2020-02-14
朱伟
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to differences in research methods and included populations, the results of the studies were not completely consistent.
At present, there is no consistent method suitable for clinical miRNA detection

Method used

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  • A plasma miRNA marker related to the auxiliary diagnosis of IGA nephropathy and its application
  • A plasma miRNA marker related to the auxiliary diagnosis of IGA nephropathy and its application
  • A plasma miRNA marker related to the auxiliary diagnosis of IGA nephropathy and its application

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Embodiment Construction

[0036] From 2013 to 2015, the inventor collected a large number of venous plasma samples from patients with IgA nephropathy and normal physical examination population from the First Affiliated Hospital of Nanjing Medical University, and finally selected 83 cases of IgA nephropathy and 82 cases of The normal control sample was used as the experimental sample for the initial screening of Exiqon miRNA qPCR panel chip and subsequent series of qRT-PCR verification. The plasma samples of selected patients were pathologically confirmed as patients with IgA nephropathy. The demographic data and clinical data of these samples were collected systematically.

[0037] Refer to the flowchart ( figure 1 ), randomly selected 20 IgA nephropathy samples and 10 normal controls from the plasma samples of IgA nephropathy and normal controls, and mixed them into 2 mixed samples of IgA nephropathy plasma and 1 normal mixed sample (one mixed sample consisted of 10 200ul plasma samples were pooled ...

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Abstract

The invention discloses a blood plasma miRNA marker related to IgA nephropathy auxiliary diagnosis and application of the blood plasma miRNA marker. The marker is one or more of miR-148a-3p, miR-150-5p, miR-20a-5p and miR-425-3p. Blood plasma miRNA has the advantages of being good in stability, minimally invasive, easy to acquire and high in sensitivity and specificity by serving as the novel biomarker. Development and utilization of the molecular marker provide a new direction for diagnosis and further treatment of various diseases including IgA nephropathy. The IgA nephropathy blood plasma miRNA marker with clinical diagnosis potential can be obtained in a more targeted mode through the research, and the reliability and the repeatability of the groups of miRNA serving as the minimally-invasive marker for diagnosing the IgA nephropathy are proved through the research.

Description

technical field [0001] The invention belongs to the field of genetic engineering nephrology, and relates to a plasma miRNA marker related to the auxiliary diagnosis of IgA nephropathy and its application. Background technique [0002] IgA nephropathy (Immunoglobulin A nephropathy, IgAN) is the most common primary glomerulonephritis, characterized by mesangial cell proliferation, increased matrix, with extensive IgA deposition. At present, IgA nephropathy must be confirmed by renal biopsy before diagnosis. But kidney biopsy is an invasive test that cannot be repeated in the same patient. Early diagnosis of IgA nephropathy has a good prognosis, but if the disease course exceeds 20 years, more than 40% of patients will enter irreversible end-stage renal failure. Therefore, the discovery of non-invasive biomarkers is of great significance for early clinical diagnosis of IgA nephropathy. [0003] MicroRNAs (miRNAs) are a class of small non-coding RNA molecules with a length of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱伟周鑫单霞齐炼文黄泽波王同杉吴晶晶朱明霞王魏魏陈彦邢昌赢
Owner 朱伟