Combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in blood sample of living body

A blood sample, LC-MS technology, applied in the field of biochemical analysis and detection, can solve the problems of questionable results accuracy, single lipid, and small amount of lipids, etc., and achieve the effect of improving detection efficiency and accuracy

Inactive Publication Date: 2016-11-09
LIAONING RUNSHENG KANGTAI BIOMEDICAL TECH CO LTD
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Problems solved by technology

[0006] The amount of lipids in the blood detected in the existing reports is small, or only a single type of lipids, and the detection results of different detection instruments used in different reports are not uniform, and most methods combine Metabolic correlation network analysis and database retrieval are used to analyze and deduce the structure of possible bi

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  • Combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in blood sample of living body
  • Combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in blood sample of living body
  • Combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in blood sample of living body

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[0050] specific implementation plan

[0051] Detection of lipid species in blood of a hemophilia patient

[0052] 1) Internal standard solution and extraction solution preparation

[0053] Internal standard solution: isopropanol-acetonitrile solution (1:1) containing 19:0-19:0 PC, 17:0-17:0 PE, 12:0 SM, 19:0 Lyso PC with a concentration of 5 μg / mL . Extraction solution: chloroform-methanol (2:1).

[0054] 2) Patient blood sample processing

[0055] For the treatment of blood samples from patients, the plasma was frozen at -80°C and thawed in a refrigerator at 4°C, and 10 μL of plasma was taken, followed by adding 10 μL of mixed internal standard solution, 10 μL of 0.9% NaCl, and 100 μL of chloroform-methanol (2:1) extract, vortexed Rotate for 20 seconds, and let stand in a refrigerator at 4°C for 30 minutes. Centrifuge at 7800g / min for 3min. Draw the lower layer liquid into the 0.5mLEP tube with a 1mL syringe, and immediately draw 40μL into the liner tube. Blow dry with...

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Abstract

The invention provides a combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in a blood sample of a living body, belonging to the field of bioanalytical chemistry. The method comprises the following steps: subjecting a to-be-detected sample to pretreatment; then adding a reaction solvent and an extraction agent for extraction; and then carrying out liquid chromatographic-mass spectroscopic detection. The method is simple to operate and high in selectivity and sensitivity, and can rapidly detect 113 lipids in blood, wherein the 113 lipids include 48 of phosphatidylcholines (PC), 21 lysophosphatidylcholines (Lyso-PC), 17 phosphatidylethanolamines (PE), 4 lysophosphatidylethanolamines (LPE), 6 lysophosphatidylinositols (PI), 9 sphingomyelins (SM) and 8 ceramides (Cer). The method has precision meeting requirements on analysis of serum samples, good stability and high detection sensitivity to most lipid metabolites, and is applicable to lipidomic analysis of serum samples.

Description

technical field [0001] The invention relates to a liquid mass spectrometry method for high-throughput detection of 113 kinds of lipids in biological blood samples, which belongs to the field of biochemical analysis and detection, and can quickly and high-throughput detect 113 kinds of lipid components in blood. The detection efficiency and accuracy are greatly improved. Background technique [0002] Blood is an important part of the human body. It flows through various organs and tissues in the human body, participates in the metabolism of the human body, regulates functions, and maintains the balance of the internal and external environment of the human body. When pathological changes occur in any part of the human body, specific biomarkers will be released into the blood. In addition, blood sample detection is non-invasive and is the most readily available biological sample that can be used as in vitro diagnosis, so blood routine is also the most direct and easy-to-obtain ...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 曹云峰孙晓宇洪沫房中则高鹏赵福荣赵欣慰董军
Owner LIAONING RUNSHENG KANGTAI BIOMEDICAL TECH CO LTD
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